'Institute of Electrical and Electronics Engineers (IEEE)'
Doi
Abstract
Integrated multimodal cross-sectional or volumetric
imaging techniques give us fruitful information to understand the
behavior or status of target objects such as biological samples. Most
of the reported systems for this purpose are either time consuming
due to scanning or use additional reference beams such as in
interferometry. Therefore, fast, simple, highly efficient, and powerful multimodal imaging systems that can perform cross-sectional
imaging with simple algorithms are worth to be investigated. In
this paper, a multimodal technique for cross-sectional quantitative
phase and fluorescence imaging with computational microscopy
is presented. We combine cross-sectional fluorescence and quantitative phase imaging by using the transport of intensity equation
(TIE) and numerical wave propagation. The amplitude and phase
of the fluorescence light wave with partially spatial coherence are
obtained from three defocused intensity patterns. The proposed
hybrid imaging system is simple, compact, and non-iterative. We
present experimental results of microbeads and fluorescent proteinlabeled living cells of the moss Physcomitrella patensto demonstrate
the performance of the proposed imaging system