Engelbrekt, Christian

Tang, Jing

Werchmeister, Rebecka Maria Larsen

Zhang, Jingdong

Enzymatic biofuel cells (EBFCs) are electrochemical devices, that produce electricity from energy stored in fuel molecules under catalysis of enzymes. An EBFC contains a bioanode and/or a biocathode, in which enzymes are used to catalyse oxidation of fuel molecules such as sugars, and dioxygen reduction, respectively. The advantage of EBFCs is to generate energy from abundant fuel molecules without using expensive noble metals. On the other hand, development of EBFCs is still at the research stage due to instability of the biocatalysts. Here, we are developing a bioanode using graphene as supporting material, polyethyleneimine (PEI) as linker and glucose oxidase (GOD) as the chosen enzyme. GOD can catalyze oxidation of glucose to gluconolactone, but needs a mediator to assist electron transfer between the enzyme and electrodes. The redox molecule ferrocene carboxylic acid (FcCOOH) is immobilized together with GOD on the bioanode. Structure and composition of the graphene-GOD bioanode are shown in Fig. 1b. Electrochemical catalytic performance of the prepared bioanode has been observed. An EBFCs with the bioanode and the commercial Pt cathode have been successfully assembled and systematically investigated. The assembled EBFCs show good reproducibility. EBFCs provide maximum output power density 2.47 μW cm-2 at 35 ℃, indicating the optimized activity of EBFCs fed with glucose

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Jul 12, 2018Graphene-glucose oxidase bioanodes for enzymatic biofuel cellsTang, Jing; Werchmeister, Rebecka Maria Larsen; Engelbrekt, Christian; Zhang, JingdongPublished in:Book of Abstracts Sustain 2017Publication date:2017Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Tang, J., Werchmeister, R. M. L., Engelbrekt, C., & Zhang, J. (2017). Graphene-glucose oxidase bioanodes forenzymatic biofuel cells. In Book of Abstracts Sustain 2017 [C-15]     Graphene-glucose oxidase bioanodes for enzymatic biofuel cells J. Tang1, R. M. L. Wercheister1, C. Engelbrekt1 and J. Zhang1,* 1 Technical University of Denmark, Department of Chemistry, 2800 Kgs. Lyngby, Denmark * Corresponding author email: jz@kemi.dtu.dk Enzymatic biofuel cells (EBFCs) are electrochemical devices, that produce electricity from energy stored in fuel molecules under catalysis of enzymes, Fig. 1a. An EBFC contains a bioanode and/or a biocathode, in which enzymes are used to catalyse oxidation of fuel molecules such as sugars, and dioxygen reduction, respectively. The advantage of EBFCs is to generate energy from abundant fuel molecules without using expensive noble metals. On the other hand, development of EBFCs is still at the research stage due to instability of the biocatalysts. Here, we are developing a bioanode using graphene [1] as supporting material, polyethyleneimine (PEI) as linker and glucose oxidase (GOD) as the chosen enzyme, Figure 1b. GOD can catalyze oxidation of glucose to gluconolactone, but needs a mediator to assist electron transfer between the enzyme and electrodes [2]. The redox molecule ferrocene carboxylic acid (FcCOOH) is immobilized together with GOD on the bioanode. Structure and composition of the graphene-GOD bioanode are shown in Fig. 1b. Electrochemical catalytic performance of the prepared bioanode has been observed, Fig. 1c. An EBFCs with the bioanode and the commercial Pt cathode have been successfully assembled and systematically investigated. The assembled EBFCs show good reproducibility. EBFCs provide maximum output power density 2.47 µW cm-2 at 35 ℃, indicating the optimized activity of EBFCs fed with glucose. From Fig.  1. Illustration of (a) an enzymatic biofuel cell and (b) the graphene-GOD bioanode. (c) Cyclic voltammetry of the graphene-GOD in the absence (black) and presence (blue) of glucose in 20 mM phosphate buffer, pH 7.0. Scan rate 10 mV/s. The graphene-GOD bioelectrode was prepared by dropcasting 20 µL graphene-PEI-FcCOOH-GOD solution synthesized by mixing 200 µL 18 mg/mL GOD and 800 µL graphene-PEI-FcCOOH ink for 20 hours at 4 °C, and then 10 µL 1.0 wt% Nafion solution on the 4.0 × 5.0 mm2 carbon paper electrode. The ink was produced by first heating 17 mL Milli-Q, 5.0 mg FcCOOH, 2.0 mL 1.0 mg/mL graphene oxide solution, and 1.0 mL 40 mg/mL PEI solution together for 60 minutes at 95 °C and concentrating to 4.0 mL. Acknowledgments Finance support from Danish Research Council (FTP-YDUN) is greatly appreciated. References [1] M. A. Raj and S. A. John, J. Phys. Chem. C. 4326, 117 (2013). [2] B. Kowalewska and K. Jakubow, Sensors. Actuat. B-Chem. 852, 238 (2017). 

Graphene-glucose oxidase bioanodes for enzymatic biofuel cells

http://orbit.dtu.dk/ws/files/140841952/Sustain2017abstract_glucose_oxidase_bioanodes_for_enzymatic_fuel_cells._Jing_Tang_final_version.pdf

Graphene-glucose oxidase bioanodes for enzymatic biofuel cells

Abstract

Similar works

Full text

Available Versions

Online Research Database In Technology