Ardenkjær-Larsen, Jan Henrik

Duus, Jens Øllgaard

Kjeldsen, Christian

Dissolution dynamic nuclear polarization (DNP) NMR can be used to increase the sensitivity of 13CNMR signal by up to four orders of magnitude. This allows for real time monitoring of reactionsand observation of intermediates. The biggest drawback of the method is the loss of polarizationwith T1 relaxation, but even with this limitation, it is possible to obtain detailed reaction parametersin less than one minute. The enzyme investigated was β-galactosidase from E. coli (E.C. 3.2.1.23). It is well described and the mechanism is generally accepted to be a double displacement with a covalently bound intermediate, however, this evidence is based on mutant of X-ray crystallography and simulations. As the natural substrate lactose does not have any quaternary carbon with long T1, the unnatural substrate o-nitrophenyl β-D-galactopyranoside was used (figure 1) as the quaternarypositions have T1 relaxations of ca. 15 s instead of &lt;2 s. The DNP NMR monitoring of the hydrolysis of this substrate can be seen in figure 2, and another use of this substrate is for optimizing the conditions for a labelled substrate (figure 1), which would further increase the signal and allow monitoring of the carbohydrate instead of the aglycon. This is, however, not commercially available and had to be synthesized from doubly labelled galactose

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DNP NMR of carbohydrate converting enzymes

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DNP NMR of carbohydrate converting enzymes

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