Poster presented at Symposium of the Pest Management Council of the Philippines. 200

Cruz, F.S. de la

Damasco, O.P.

Dela Cueva, F.M.

Dinglasan, E.G.

Dizon, T.O.

Molina, A.B.

English

Cueva, F.M. de la

CGSpace (CGIAR)

Role of Healthy-Looking Banana and Alternate Hosts in the Spread of Banana Bunchy Top Disease (BBTD)INTRODUCTIONBanana bunchy top nanovirus (BBTV), the cause of banana bunchy top disease (BBTD), is the most important virus affecting banana agriculture. BBTV can persist naturally in banana plants causing no apparent symptoms of the disease or the plants remain healthy-looking and asymptomatic. Furthermore, alternate hosts of BBTV outside Musaceaefamily can occur experimentally.These healthy-looking banana plants and alternate hosts can have an important role on the spread of BBTD by acting and serving as a reservoir of BBTV.OBJECTIVE• To determine the important role of healthy-looking banana and alternate hosts in the spread of banana bunchy top disease (BBTD).FM Dela Cueva1, EG Dinglasan1, TO Dizon1, FS Dela Cruz1, OP Damasco1, LA Gueco1, AB Molina21 IPB-CSC, CA, UPLB; 2 Bioversity InternationalMETHODOLOGYHealthy-looking bananasELISAInsect transmission to tissue-cultured LakatanA.   Insect transmission of healthy-looking banana plantsB.   Insect transmission of BBTV to alternate hostsViruliferousPentalonia nigronervosaAlternate host (e.g. Dieffenbachia sp.)ELISABBTV-infected bananaISOLATES/CULTIVARBBTV% infectionELISAInsect TransmissionBatangasBungulan + + 10Saba + - 0CaviteBungulan + + 60Dwarf Cavendish - - 0Latundan - - 0Saba + - 0LagunaBungulan + - 0Lak-Min (Saba) + + 20Lakatan + + 100QuezonSaba - + 50Saba + - 0Table 1.  Result of insect transmission of healthy-looking banana to tissue-cultured Lakatan.B.   Insect transmission of BBTV to alternate hostsInitial studies, based on insect transmission and ELISA, show three possible and potential plant reservoir of BBTV outside Musaceae family. RESULTSA.   Insect transmission of healthy-looking banana plantsFigure 1. BBTV infection 3-4 weeks after insect transmission.Characteristic symptoms of BBTV infection are marginal chlorosis, leaf narrowing & reduction in surface area, rosetting & bunchy top.► ELISA & insect transmission results indicate the presence of BBTV from healthy-looking plants and that the virus can be transferred to other plants, respectively.► Negative to ELISA and insect transmission could be due to low titer of the virus and efficiency of transmission.Assay Hosts % infection ELISA*Colocasia bicolor 10 1/10 (0.390)Costus sp 0 0/4Dieffenbachia sp 0 0/4Colocasia sp 1 0 0/4Colocasia sp 2 0 0/5Canna sp 0 2/9 (0.403; 0.442)Heliconia sp  25 3/4 (0.371; 0.398; 0.362)Camia 0 0/1Banana cv Lak-Dav 86.71 2/2 (0.363; 0.371)* Number of plants detected (+) / total number of plants assayed;                     absorbance at 405 nm: H – 0.181; (+) – 0.724.Symptoms on alternate hosts started to appear 4 weeks post-insect transmission only on variegated gabi and Heliconia sp as marginal chlorosis and mosaic, respectively. Bandera Espanola remained asymptomatic.CONCLUSION• Asymptomatic & apparently healthy-looking banana plants & other alternate hosts outside Musaceae family can act as reservoir to BBTV and can serve as foci of infection on the spread of BBTD.GEERING, A. D. W. & THOMAS, J. E. 1997. Search for alternative hosts of banana bunchy top virus in Australia. Australasian Plant Pathology 26: 250-254.RAM, R. D. & SUMMANWAR, A. S. 1984. Colocasia esculenta (L.) Schott. A reservoir of bunchy top disease of banana. Current Science 53: 145-146.SMITH, M. C., HOLT, J., KENYON, L. & FOOT, C. 1998. Quantitativeepidemiology of banana bunchy top virus disease and its control. Plant Pathology 47: 177-187.LITERATURE CITED:

Role of healthy-looking banana and alternate hosts in the spread of banana bunchy top disease

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Role of healthy-looking banana and alternate hosts in the spread of banana bunchy top disease

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