Gizachew, D.

Grace, Delia

Hanson, Jean

Szonyi, Barbara

Tegegne, Azage

Ellis R. John

Ditzel

Ellis

Hemmingsen

Crossref

Chaperonins

R. John, Ellis

Elsevier - Publisher Connector 

Chaperonins 

MUCC (Crossref)

CGSpace (CGIAR)

Aflatoxin analysis of dairy feeds and milk in the Greater Addis Ababa milk shed, Ethiopia   Dawit Gizachew, Barbara Szonyi, Azage Tegegne, Jean Hanson and Delia Grace   Consultative workshop on milk quality Addis Ababa, Ethiopia 25-26 June 2015   Aflatoxins • Toxic secondary metabolites produced by Aspergillus fungi • Contaminate a variety of foods such as corn, oil seed and animal feed  • One of the most toxic forms of aflatoxin (AFB1) is converted to AFM1 and excreted in milk by lactating animals that consume contaminated feed • Highly carcinogenic; cause liver cancer, stunting and immunosuppression Aspergillus flavus  (Maize breeding program at Texas A&M University)  Effect of aflatoxins on human health  Liver of a rat fed with high doses of aflatoxin B1  Notice the induced tumours in the liver (http://www.ansci.cornell.edu) AFB1 is potent carcinogen. It can cause liver cancer Aflatoxin Regulatory Guidance EU  0.05 ppb 4 ppb Study locations: the greater Addis Ababa milk shed   Sululta Sebeta Debre Zeit IDDELS (http://www.ideels.uni-bremen.de/highlands.html) • Includes Addis Ababa, Debre Zeit, Sebeta, Sendafa and Sululta • It serves as a major supplier in and around Addis Ababa • The sector is commercial and uses concentrate feeding Feed analysis of aflatoxin B1 (AFB1) using enzyme-linked immunosorbent assay (ELISA) Study methods • Study participants: • 100 dairy farmers  • 27 from Addis Ababa, 23 from Debre Zeit, 9 from Sebeta, 31 from Sendafa and 10 from Sululta • 5 feed producers  • 5 feed processors • 9 feed traders • A semi-structured questionnaire was administered to all study participants • 100 gram samples of each feed type were collected All dairy farmers used concentrates every day to feed cattle of all ages  •Ingredients in concentrates feed include: •Wheat barn (100%) •Noug seed cake (73%) •Pea hulls (37%) •Maize grain (12%)   Noug cake W Pea hulls and wheat bran Results of feed analysis Summary of aflatoxin (AFB1) contamination of feed  in the value chain (N=156)  aNumber of contaminated samples bPercentage of AFB1 contaminated feed samples cThe highest AFB1 concentration was 419 ppb from milk producer  Results of feed analysis 050100150200250300350400Noug cake Wheat bran Maize grain Brewer's dryyeastSample               Mean ± SD (ppb) Noug cake                362 ± 38 Wheat bran               15 ± 6 Maize grain              18 ± 11 Brewer's dry yeast    15 ± 4   AFB1 (ppb) Concentration of AFB1 (ppb) in individual dairy feed ingredients  Gizachew, D., et. al., Food Control (2015)  The fate of wheat bran and noug cake in the peri-urban dairy value chain Noug Seed (Guizotia abyssinica) Beside its use as oil seed and animal feed, noug is sold in the local market for consumption.   Milk analysis of aflatoxin M1 (AFM1) using enzyme-linked immunosorbent assay (ELISA) Conversion of AFB1 to AFM1 Animals, under the influence of the cytochrome P450 oxidase system found in their microflora and own cells, hydroxylate aflatoxin B1 (AFB1) to aflatoxin M1 (AFM1)    Study Methods • Study participants: • 100 dairy farmers  • 27 from Addis Ababa, 23 from Debre Zeit, 9 from Sebeta, 31 from Sendafa and 10 from Sululta • 10 milk collectors • A semi-structured questionnaire was administered to all study participants • 50 ml of raw milk samples were collected Results of milk analysis from milk producers and collectors aNumber of contaminated samples bPercentage of AFM1 contaminated milk samples cThe highest AFM1 concentration was 4.98 ppb from milk producer.  Summary of aflatoxin (AFM1) contamination of milk  in the value chain (N=110)  Gizachew, D., et. al., Food Control (2015)  Aflatoxin contamination level in milk in some African countries Gizachew, D., et. al., Food Control (2015)  Conclusion • Noug (Guizotia abyssinica) cakes are widely used in the greater Addis Ababa milk shed as cattle feed and have been found to be contaminated with AFB1 • Intervention studies, including chemical detoxification of the feed, might be necessary to minimize the aflatoxin contamination along the value chain • Together with the Ministry of Health, there is need to study aflatoxin in human urine and breast milk to examine the impact on human health • Further studies are required on the impact of aflatoxin on human and animal health in Ethiopia      This work is financed by the CGIAR Research Program on Agriculture for Nutrition and Health and the Livestock and Irrigation Value chains for Ethiopian Smallholders (LIVES) project.  It is implemented in a partnership with LIVES, Genebank and the Food Safety and Zoonoses program at ILRI.  It contributes to the CGIAR Research Program on Agriculture for Nutrition and Health. Acknowledgements  The presentation has a Creative Commons licence. You are free to re-use or distribute this work, provided credit is given to ILRI. better lives through livestock ilri.org 

Aflatoxin analysis of dairy feeds and milk in the Greater Addis Ababa milk shed, Ethiopia

https://doi.org/10.1016/s0960-9822(99)80223-1

Chaperonins

Abstract

Similar works

Full text

Available Versions

Crossref

Elsevier - Publisher Connector

Elsevier - Publisher Connector

MUCC (Crossref)

CGSpace (CGIAR)

MUCC (Crossref)