Carulla, Juan E.

Cortés, J.E.

Hess, H.D.

Kreuzer, M.

Lascano, Carlos E.

Pabón, M.L.

Tiemann, Tassilo T.

Lascano Aguilar, Carlos Eduardo

CGSpace

A joint project with:Swiss Centre for International AgricultureSchweizerisches Zentrum für Internationale LandwirtschaftCentre Suisse pour l’Agriculture InternacionalThe financial support of the Swiss Centre for International Agriculture (ZIL) is gratefully acknowledged.Protein digestibility of tannin-containing forages in the rumen and the abomasum as determined in vitroH.D. Hess1,2, J. Cortés3, J.E. Carulla3, Martha L. Pabón3, T.T. Tiemann2,4, C.E. Lascano4 and M. Kreuzer21Agroscope Liebefeld-Posieux, Swiss Federal Research Station for Animal Production and Dairy Products (ALP); 2Institute of Animal Science, ETH-Zurich; 3Universidad Nacional de Colombia, Bogotá; 4Centro Internacional de Agricultura Tropical (CIAT), Cali, ColombiaBackground¾The Centro Internacional de Agricultura Tropical identified a range of tropical shrub legume species with high crude protein (CP) content and good growth characteristics.¾The feeding value of certain species may be limited by high concentrations of tannins.¾Tannins decrease the ruminal degradation of crude protein. ¾A significant proportion of this rumen-protected protein could be liberated and degraded in the abomasum. Materials and MethodsExperimental diets¾Thirteen legume supplemented diets. ¾Basal substrate: Brachiaria humidicola 6133.¾Legume supplement (1/3 of dietary dry matter [DM])either one of the following legumes aloneCratylia argentea 18516 Leucaena leucocephala 734Flemingia macrophylla 17403 Calliandra calothyrsus 22310C. callothyrsus 22316or combinations of the non-tanniniferous Cratylia withthe other legumes in proportion of 2:1 and 1:2.In vitro technique¾Digestibility technique modified to determine ruminal and abomasal degradability of CP.¾Nine samples per diet incubated in ruminal fluid and McDougall buffer (1:4) for 48 h.¾Determination of ammonium concentration in fermentation fluid after 0, 6, 12 and 48 h.¾Three samples filtered for estimation of apparent degradability of DM.¾Three samples treated with neutral detergent solution for estimation of true ruminal DM and CP degradation.¾Three samples further incubated with HCl-pepsin for estimation of apparent abomasaldigestibility of the rumen-undegraded DM and CP.Conclusions¾Tanniniferous legumes decreased ruminal CP degradation and ammonium production and increased the proportion of dietary CP degraded during acid-pepsin incubation.¾A major part of the tannin-bound protein, which is protected from microbial degradation in the rumen, is available for acid-pepsin digestion in the abomasum.¾The use of mixtures of legumes with and without tannins could effectively improve the supply of metabolically available protein to the animal.ResultsIn vitro fermentation systems are an important tool in assessing the feeding value of tanniniferous legumes.Ammonium production (micromol/h)04812162024Cratylia 2:1 1:2 0:3 2:1 1:2 0:3 2:1 1:2 0:3 2:1 1:2 0:3Legume ratioLeucaenaFlemingiaCalliandra22310 22316In vitro  dry matter digestibility (mg/g)0200400600800Cratylia 2:1 1:2 0:3 2:1 1:2 0:3 2:1 1:2 0:3 2:1 1:2 0:3Legume ratioLeucaenaFlemingiaCalliandra22310 22316True ruminal CP degradability (mg/g CP supply)02004006008001000Cratylia 2:1 1:2 2:1 1:2 0:3 2:1 1:2 0:3 2:1 0:3Legume ratioLeucaenaFlemingiaCalliandra22310 22316Acid-Pepsin digested CP (mg/g CP supply)050100150200250300350Cratylia 2:1 1:2 2:1 1:2 0:3 2:1 1:2 0:3 2:1 0:3Legume ratioLeucaenaFlemingiaCalliandra22310 22316

Protein digestibility of tannin-containing forages in the rumen and the abomasum as determined in vitro

Pabón, ML

https://cgspace.cgiar.org/bitstream/10568/57808/1/pub_HessHD_2006_16090.pdf

Protein digestibility of tannin-containing forages in the rumen and the abomasum as determined in vitro

Abstract

Similar works

Full text

Available Versions

CGSpace

CGSpace