Almeida, Diana

Andrade, José Carlos

Barbosa, Joana Cristina

Costa, Francisca

Fonseca, Mariana

Freitas, Ana Cristina

Gomes, Ana Maria

Machado, Daniela

English

Repositório Institucional da Universidade Católica Portuguesa

Aggregation properties of probiotic strains under aerobic and anaerobic conditionsDaniela Machado a, José Carlos Andrade b, Joana Cristina Barbosa a,Francisca Costa a, Mariana Fonseca a, Diana Almeida a, Ana Cristina Freitas a†,Ana Maria Gomes aa Universidade Católica Portuguesa, CBQF – Centro de Biotecnologia e Química Fina – Laboratório Associado, EscolaSuperior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugalb TOXRUN – Toxicology Research Unit, University Institute of Health Sciences, CESPU, CRL, 4585-116 Gandra, PortugalObjectivesAggregation between microorganisms from the same species (auto-aggregation) and from different species (co-aggregation) isconsidered a desirable property of probiotics, since it has been related with successful gut colonisation by probiotic strains andclearance of intestinal pathogens, respectively.This study aimed to evaluate the auto- and co-aggregation properties of the novel probiotic candidate Akkermansia muciniphila DSM22959 and the commercial probiotics Bifidobacterium animalis subsp. lactis BB-12 and Lacticaseibacillus rhamnosus GG (formerlyclassified as Lactobacillus rhamnosus), with the pathogens methicillin-resistant Staphylococcus aureus [MRSA] and Escherichia coliO157:H7, under two atmospheric conditions (aerobiosis and anaerobiosis).MethodsInulin + Riboflavin(65±5 mg)(63±6 mg)ResultsConclusionThis work provides novel insights regarding aggregation properties of the novel probioticcandidate A. muciniphila DSM 22959 and commercial probiotics (B. animalis subsp. lactis BB-12 and L. rhamnosus GG) under two atmospheric conditions. Furthermore, the demonstratedaggregation properties of A. muciniphila DSM 22959 support its use as probiotic.References1. Andrade J. C. et al. Front Bioeng Biotechnol. 2020; 8:550.2. Collado M.C. et al. Eur Food Res Technol. 2008; 226: 1065–1073.3. Jena, P.K. et al. Microbiol Immunol. 2013;57(6):407-16.AcknowledgementsThis work was supported by national funds through FCT/MEC (PIDDAC), project references IF/00588/2015, under the Scientific Employment Stimulus - Individual Call (CEEC Individual) -CEECIND/00520/2017/CP1404/CT0001, and by Operational Program Competitiveness and Internationalization in its FEDER component and by the budget of the Foundation for Science and Technology, I.P.(FCT, IP) in its OE component, project reference POCI-01-0145-FEDER-031400-PTDC/BAA-AGR/31400/2017. We would also like to thank the scientific collaboration under the FCT project UIDB/50016/2020.Figure 1. Auto-aggregation ability of A. muciniphila DSM 22959 (blue), B.animalis subsp. lactis BB-12 (orange) and L. rhamnosus GG (grey) underaerobic (a) and anaerobic (b) conditions.Main Findings:Auto-aggregationa) b)Co-aggregation with pathogensa) b)Figure 2. Co-aggregation ability of: A. muciniphila DSM 22959 with S. aureus (dark blue) and E. coli (lightblue); B. animalis subsp. lactis BB-12 with S. aureus (dark orange) and E. coli (light orange); L. rhamnosusGG with S. aureus (dark grey) and E. coli (light grey) under aerobic (a) and anaerobic (b) conditions.- All tested probiotic strainsshowed auto-aggregationabilities, and also capacity toco-aggregate with pathogensat all time-points and underboth atmospheres.- Overall, these aggregationproperties increased withincreasing incubationperiod.• A. muciniphilaDSM 22959• B. animalis subsp. lactis BB-12• L. rhamnosus GGProbiotics Pathogens• MRSA S. aureus• E. coli O157:H7AnaerobicAerobicBacterial growth (37 oC, 24h)Spectrophotometric methodAuto- and co-aggregation determinationAuto- Co-AggregationProbiotic strains Probiotic PathogenMethodsAbsorbance at 600 nm was measured periodicallyat the top of the culture suspensionBacteria were allowed to aggregate in aerobic andanaerobic atmospheresTime-points: 2, 4, 20and 24 hoursAggregation assayswere carried out twiceindependently withtechnical duplicates.

Aggregation properties of probiotic strains under aerobic and anaerobic conditions

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Aggregation properties of probiotic strains under aerobic and anaerobic conditions

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