Brito, Margarida

Ferreira, Catarina

Fialho, Arsénio

Gomila, Margarita

Henriques, Isabel

Lameiras, Catarina

Manaia, Célia M.

Mil-Homens, Dalila

Rocha, Jaqueline

English

Repositório Institucional da Universidade Católica Portuguesa

CONGRESS OF MICROBIOLOGY AND BIOTECHNOLOGY 2019December 5th-7th, 2019University of Coimbra (Pólo II)BOOK OF ABSTRACTS121 I1. Environmental Microbiology and Biotechnology P57. Do Klebsiella pneumoniae environmental strains maintain clinically relevant genomic and phenotypic traits? Jaqueline Rocha1, Catarina Ferreira1, Dalila Mil-Homens2, Margarida Brito3, Catarina Lameiras4, Arsénio Fialho2, Isabel Henriques5,6, Margarita Gomila7, Célia M. Manaia1 1 Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Porto, Portugal 2 iBB-Institute of Bioengineering and Biosciences, Instituto Superior Técnico, Lisbon, Portugal 3 Serviço de Virologia IPOP-FG, Porto, Portugal 4 Serviço de Microbiologia IPOP-FG, Porto, Portugal 5 Department of Life Sciences, Faculty of Science and Technology, University of Coimbra, Coimbra, Portugal 6 CESAM, University of Aveiro, Aveiro, Portugal 7 Microbiologia, Departament de Biologia, Universitat de les Illes Balears, Palma de Mallorca, Spain E-mail: jrocha@porto.ucp.ptExtended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae are well-known pathogens, increasingly reported in the environment. This fact represents a human health concern since third-generation cephalosporins are frontline antibiotics used to treat infections caused by this species. A major question is whether environmental ESBL-producing K. pneumoniae can infect humans. To address this question, this study compared clinical and environmental K. pneumoniae strains regarding genetic and phenotypic traits and assessed their potential infectious capacity. Therefore, 59 isolates (25 environmental and 34 clinical) of cefotaxime-resistant K. pneumoniae were characterized based on antibiotic resistance phenotype, plasmids content, and horizontal gene transfer capacity. A subset of these isolates was tested for infection capacity in Galleria mellonella (23 environmental and 24 clinical) and for whole genome sequencing (7 environmental and 11 clinical). Most environmental (80%, 20/25) and clinical isolates (94%, 32/34) were multidrug resistant. Environmental isolates presented mostly 2 plasmids (48%, 12/25) while clinical isolates presented 1 or 2 plasmids (41%, 14/34 each), however bacterial conjugation was more frequent among clinical (76%, 26/34) than environmental isolates (40%, 10/25). G. mellonella health index was lower after infection with clinical (most of the infected isolates scored 1) than with environmental isolates (most of the infected isolates scored 6). A screening of the whole genome sequences, made in parallel with data available in public databases (in total 73 environmental and 78 clinical), targeting 6 groups of genes related to antibiotic and metal resistance, virulence, efflux systems, oxidative stress and quorum sensing, evidenced the existence of 1383 gene variants. A total of 438 genes out of the 1383 were common to all isolates, while 460 and 485 genes were found exclusively in environmental and in clinical isolates, respectively. A screening of the whole genome-deduced amino acid sequences demonstrated a common putative proteome, related with all functional categories, in environmental and clinical isolates (n=2715), although the number of exclusive amino acid sequences was higher for clinical isolates (n=577 in clinical vs. n=205 in environmental). These results suggest the adaptation of K. pneumoniae to environmental or clinical niches, although highlight that putative clinically relevant traits may persist in bacteria thriving in the environment. 

Do Klebsiella pneumoniae environmental strains maintain clinically relevant genomic and phenotypic traits?

https://repositorio.ucp.pt/bitstream/10400.14/29758/1/P57.%20Do%20Klebsiella%20pneumoniae%20environmental%20strains%20maintain%20clinically%20relevant%20genomic%20and.pdf

Do Klebsiella pneumoniae environmental strains maintain clinically relevant genomic and phenotypic traits?

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Repositório Institucional da Universidade Católica Portuguesa