Abreu-Silva, Joana

Ferreira, Catarina

Manaia, Célia M.

Vaz-Moreira, Ivone

English

Repositório Institucional da Universidade Católica Portuguesa

CONGRESS OF MICROBIOLOGY AND BIOTECHNOLOGY 2019December 5th-7th, 2019University of Coimbra (Pólo II)BOOK OF ABSTRACTS73 I1. Environmental Microbiology and Biotechnology P9. Dynamics of carbapenem resistance in urban wastewater treatment plants and receiving water bodies: drivers and opportunities for action Joana Abreu-Silva1*, Catarina Ferreira1*, Ivone Vaz-Moreira1, Célia M. Manaia1 1Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Porto, Portugal *These authors contributed equally to this workE-mail: jsilva@porto.ucp.ptUrban wastewater treatment plants (UWTPs) are described as reservoirs of antibiotic resistance. It has become increasingly clear that antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) released by well-functioning UWTPs may have a negative impact on the environment, representing a threat for humans and/or ecosystems. Assessing risks where the environment and humans meet is therefore critical. This study has focused on antibiotic resistance loads and the epidemiological dynamics of resistance in natural water bodies receiving treated urban wastewaters. Wastewater samples were collected from 4 full-scale UWTPs (PT1-PT4) located in Northern Portugal, at different stages of wastewater treatment, and river water samples were collected upstream and downstream of each treatment plant. All UWTPs have primary and secondary treatments, with PT1- PT3 reporting a tertiary treatment and PT2 an additional step of ozonation. PT4 reported the reception of hospital effluents. Samples collected in early Summer (SC1) and in early Autumn (SC2) were processed for total DNA extraction and isolation of cultivable bacteria resistant to meropenem or cefotaxime. Carbapenem resistance genes (blaKPC, blaOXA-48, and blaOXA-58), class 1 integron- integrase (intI1) and 16S rRNA were quantified based on quantitative PCR. The abundance of ARGs and intI1 (per mL of sample) in the final effluent of all UWTPs could be ranked as intI1>blaOXA-58>blaOXA-48>blaKPC, with a higher abundance of 16S rRNA gene, intl1, blaOXA-48 and blaOXA-58 observed in PT1. In PT2, blaKPC was not detected after secondary treatment and ozonationpromoted a reduction of 1.44 to 3.85 log-units in genes abundance. Comparing river water upstream anddownstream the UWTPs, the abundance of blaKPC, blaOXA-48 and blaOXA-58 genes was higher downstream PT4,PT2 and PT3 in SC2, respectively. The genes blaKPC-2, blaCTX-M-15 and intI1 were detected in isolates(Klebsiella quasivariicola) from a secondary effluent of PT3 and downstream the treatment plant (SC1). Inaddition, blaKPC-3 and intI1 were detected in 3 isolates (2 Klebsiella pneumoniae, and 1 Klebsiella variicola)after secondary and tertiary treatment from PT2, and downstream PT4 (SC1). The results demonstrate thatalthough UWTPs are effective for removal of clinically relevant ARGs, some can persist in the downstreamenvironment and associated with opportunistic pathogens.

Dynamics of carbapenem resistance in urban wastewater treatment plants and receiving water bodies: drivers and opportunities for action

https://repositorio.ucp.pt/bitstream/10400.14/29664/1/P9.%20Dynamics%20of%20carbapenem%20resistance%20in%20urban%20wastewater%20treatment%20plants%20and%20receiving.pdf

Dynamics of carbapenem resistance in urban wastewater treatment plants and receiving water bodies: drivers and opportunities for action

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Repositório Institucional da Universidade Católica Portuguesa