Almeida, A,

Coscueta, E,

Freire, C. S. R,

Moreirinha, C,

Pintado, M. M,

Ramos, P. A. B.

Santos, S. A. O.

Silva, A. M. S,

Silva, S,

Silvestre, A. J. D.

English

Repositório Institucional da Universidade Católica Portuguesa

CPH2 Health The health-promoting potential of Salix spp. bark: from the phenolic composition to the in vitro bioactivity Ramos P A B,a,b Moreirinha C,a Santos S A O,a Silva S,c Coscueta E,c Almeida A,d Pintado M M,c Freire C S R,a Silva A M S,b Silvestre A J Da a) CICECO – Aveiro Institute of Materials and b) QOPNA & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro,3810-193 Aveiro, Portugal; c) Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, 4202-401 Porto, Portugal; d) Biology Department and CESAM – Centre for Environmental and Marine Studies, University of Aveiro, 3810-193 Aveiro, PortugalEmail: patriciaaramos@ua.pt Salix spp. (Salicaceae) cultivation has attracted attention, not only as source of biomass for energy generation, but also as source of extracts with health benefits.1 Although salicin usually represents the marker bioactive of Salix spp. bark extracts, their flavanones and proanthocyanidins also exhibit interesting biological properties.1 However, the phenolic composition and related bioactivity of Salix spp. bark extracts is still underexploited, particularly regarding to S. atrocinerea Brot., S. viminalis L. and S. fragilis L.,2 whose knowledge may prompt innovative nutraceutical applications. Therefore, the present work aims at adding value to S. atrocinerea Brot., S. viminalis L. and S. fragilis L. bark, through the detailed characterization of phenolic compounds, by UHPLC-DAD-MSn analysis, and the evaluation of the in vitro antioxidant, anti-hypertensive and antibacterial effects of their extracts (Scheme 1). Thirteen phenolic compounds, including 2 acetophenones and 2 proanthocyanidins were detected. S. atrocinerea Brot. extracts revealed the highest DPPH• and ABTS+• scavenging effects, as well as inhibitory activity on angiotensin I-converting enzyme. Considering the 24 h-inhibitory effect against Staphylococcus aureus growth, extracts of S. fragilis L., S. viminalis L. and S. atrocinerea Brot. bark were bactericidal at 1250 µg mL-1. Phenolic compounds may be associated to the bioactivity of Salix spp. bark extracts. In sum, these promising insights can contribute for envisaging the sustainable and integrative use of the studied Salix spp. bark extracts, in the spite of the biorefinery concept. Scheme 1: Salix spp. bark valorization, via phenolic characterization and the in vitro bioactivity evaluation. Acknowledgements: FCT/MCTES is acknowledged for financial support to CICECO (UID/CTM/50011/2019), to QOPNA (UID/QUI/00062/2019), to CBQF (UID/Multi/50016/2019) and to CESAM (FCT UID/MAR/LA0017/2019), through national funds; co-financing, where applicable, come from FEDER within the PT2020. C. Freire also thanks FCT/MCTES for her contract under Stimulus of Scientific Employment 2017 (CEECIND/00464/2017). S. Santos and C. Moreirinha acknowledge the “AgroForWealth” project (CENTRO-01-0145-FEDER-000001) for respectively the contract and post-doctoral grant, and P. Ramos acknowledges the “MultiBiorefinery” project (POCI-01-0145-FEDER-016403) for her post-doctoral grant. The authors also thank The Navigator Company and RAIZ for providing Salix spp. samples. References: 1. a) Dou J.; Galvis L.; Holopainen-Mantila U.; Reza M.; Tamminen T.; Vuorinen T. ACS Sustain. Chem. Eng. 2016, 4, 3871. b)Freischmidt A.; Jürgenliemk G.; Kraus B.; Okpanyi S. N.; Müller J.; Kelber O.; Weiser D.; Heilmann J. Phytomedicine 2012, 19, 245.2. Ramos P. A. B.; Moreirinha C.; Santos S. A. O.; Almeida A.; Freire C. S. R.; Silva A. M. S.; Silvestre A. J. D. Ind. Crops Prod. 2019, 132, 245.92

The health-promoting potential of Salix spp. bark: from the phenolic composition to the in vitro bioactivity

https://repositorio.ucp.pt/bitstream/10400.14/28055/1/The%20health-promoting%20potential%20of%20Salix%20spp.pdf

The health-promoting potential of Salix spp. bark: from the phenolic composition to the in vitro bioactivity

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