Andrade, José Carlos

Cardoso, Beatriz

Fontes, Ana Luiza

Gomes, Ana Maria

Pimentel, Lígia

Rodriguéz-Alcalá, Luís Miguel

Salsinha, Ana Sofia

English

Repositório Institucional da Universidade Católica Portuguesa

84  Microbiotec’17 – Book of Abstracts Bioprocess Engineering  P-005 - CHARACTERIZATION OF POTENTIAL CLA-PRODUCING STRAINS ACCORDING TO LA TOLERANCE  Ana Luiza Fontes1; Lígia Pimentel1,2,3; Ana Sofia Salsinha1; Beatriz Cardoso1,4; José Carlos Andrade4; Luis Miguel Rodríguez-Alcalá1; Ana Maria Gomes1  1 - Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia; 2 - CINTESIS – Centro de Investigação em Tecnologias e Sistemas de Informação em Saúde, Faculdade de Medicina da Universidade do Porto; 3 - QOPNA – Unidade de Investigação de Química Orgânica, Produtos Naturais e Agroalimentares, Universidade de Aveiro; 4 - Centro de Investigação em Ciências da Saúde (CICS), Instituto Superior de Ciências da Saúde –Norte, CESPU Background  Conjugated linoleic acid (CLA) isomers are naturally produced from dietary linoleic acid (LA) by ruminal bacteria. However, strains of lactobacilli, bifidobacteria and propionibacteria have also demonstrated the ability to produce those bioactive fatty acids. In vitro studies normally test CLA production at 0.5 mg/mL of LA, but possibly some strains can tolerate higher concentrations and if they are producers, CLA yields may probably be higher. This work aims to determine the maximum LA concentration that potential CLA-producing strains can tolerate in an in vitro production assay.  Method  Thirty five lactobacilli, 17 bifidobacteria, 1 propionibacterium and 1 lactococcus strains were submitted to a modified method described by Roméro-Pérez et al. [1]. Activated strains were inoculated in MRS agar plates containing 1, 2 or 5 mg/mL of LA for 48 h at 37 ºC. Strains from the agar plate at the highest LA tolerated concentration were then spiked into MRS broth with LA in a 96-well microplate and subsequently incubated at 37 ºC for 48 h; growth curves were recorded at 600, 620 and 660 nm in a microplate reader. Cultures without substrate were used as control. Agar plates experiment was carried out in duplicate and microplate experiment in triplicate.  Results & Conclusions  Among the strains tested, 18 (~33%) could not grow at the lowest LA concentration assayed, having considered their tolerance below 1 mg/mL. A group of 16 strains showed growth at 1 mg/mL of LA, 8 tolerated up to 2 mg/mL and 12 up to 5 mg/mL. At 5 mg/mL lactobacilli was the dominant group, whereas at 2 mg/mL was bifidobacteria. At <1 mg/mL and 1 mg/mL the distribution of lactobacilli and bifidobacteria was similar. The propionibacterium and the lactococcus strains could grow a 1 mg/mL. In conclusion, potential CLA-producing strains exhibit different LA tolerance degrees. This is a parameter to consider in future production tests.  References & Acknowledgments  [1]      G.A. Romero-Pérez, R. Inoue, K. Ushida, T. Yajima, Biosci. Biotechnol. Biochem. 77 (2013) 648–650.  This work was financed by National funds via FCT – Fundação para a Ciência e a Tecnologia, under the project “Pro-TECh-CLnA - Microbial Production of Bioactive Conjugated Linolenic Acid Isomers to Obtain Functional Ingredients and Foods” reference PTDC/AGR-TEC/2125/2014. Financial support for the authors A.L. Fontes and L.L. Pimentel was provided by fellowships SFRH/BD/117721/2016 and SFRH/BPD/119785/2016, respectively, granted by the Portuguese government through FCT.   Keywords: LA tolerance, Probiotics, CLA, Lactic acid bacteria, Bifidobacterium   

Characterization of potential CLA-producing strains according to LA tolerance

https://repositorio.ucp.pt/bitstream/10400.14/26126/1/BookofAbstracts_15_12_2017-90-90.pdf

Characterization of potential CLA-producing strains according to LA tolerance

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Repositório Institucional da Universidade Católica Portuguesa