Morais, Rui

Pintado, Manuela

Vasconcelos, Helena

English

Repositório Institucional da Universidade Católica Portuguesa

283  Microbiotec’17 – Book of Abstracts Health Microbiology and Biotechnology P-203 - SCREENING OF ANTIMICROBIAL ACTIVITY OF THE EXOPOLYSACCHARIDE OF PORPHYRIDIUM CRUENTUM  Helena Vasconcelos1; Manuela Pintado1; Rui Morais1  1 - Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia  Background  Porphyridium sp. is a microalgae that produces and excretes a sulphated polysaccharide, the exopolysaccharide (EPS), into the culture medium. It is known that polysaccharides may have biological activity as anti-inflammatory, antibacterial and antiviral. The aim of this work was to evaluate the biological activity of EPS. The antimicrobial activity of EPS was tested against 14 microbial strains. Several tests were carried out, namely bacterial antibacterial, antifungal and antibiofilm capacity. [1, 2]. Method  EPS was tested at concentrations from 0.5% to 4%, in order to evaluate the anti-bacterial activity against several microorganisms, namely Escherichia coli, Bacillus cereus, Salmonella enteritidis, Listeria innocua, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Staphylococcus aureus methylcyllin-resistant (MRSA), Staphylococcus aureus (MSSA), Candida albicans Malassezia furfur, Malassezia inova and Malassezia sympodialis. To test the antifungal activity, Microsporum canis and Tricophyton rubrum were used. These microorganisms were cultured in a medium selective for dermatophytes for 14 days at 30 º C. In order to test the anti-bacterial and antifungal activity of EPS, the plaque microdilution method was used at minimum inhibitory concentrations (MIC). Results & Conclusions  The results obtained suggest that the EPS of Porphyridium cruentum has potential to be used as a natural bacteriostatic. However, further microbiological studies will be needed to understand the mechanism of action. It was observed that at the concentration of 4%, EPS exerted a greater inhibitory activity in the 24 hours of incubation, although not very important for the majority of the microorganisms, reaching a maximum of 11% for MRSA, followed by two Gram negative, S. enteritidis (9%) and E. coli (7%) and fungus C. albicans (6%). Pseudomonas aeruginosa did not show any inhibition in any medium in the presence of EPS. It is important to note that as the concentration of EPS decreases the percentage of inhibition of S. aureus increases. 0.5% EPS poorly inhibited S.enteritidis, E.coli and B.cereus.  The results obtained suggest that the EPS of Porphyridium cruentum has potential to be used as a natural bacteriostatic.  References & Acknowledgments   1. de Jesus Raposo, M. F., de Morais, A. M. M. B., & de Morais, R. M. S. C. (2014). Influence of sulphate on the composition and antibacterial and antiviral properties of the exopolysaccharide from Porphyridium cruentum. Life sciences, 101(1), 56-63. 3. Silva, S., Costa, E. M., Mendes, M., Morais, R. M., Calhau, C., & Pintado, M. M. (2016). Antimicrobial, antiadhesive and antibiofilm activity of an ethanolic, anthocyanin‐rich blueberry extract purified by solid phase extraction. Journal of applied microbiology, 121(3), 693-703.   Keywords: P. cruentum, EPS, antimicrobial, bioactivity 

Screening of antimicrobial activity of the exopolysaccharide of porphyridium cruentum

https://repositorio.ucp.pt/bitstream/10400.14/26204/1/BookofAbstracts_15_12_2017-289-289.pdf

Screening of antimicrobial activity of the exopolysaccharide of porphyridium cruentum

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