Faria, Joaquim

Fernández-Ibáñez, Pilar

Manaia, Célia M.

Moreira, Nuno

Narciso-Da-Rocha, Carlos

Nunes, Olga

Pastrana-Martínez, Luisa

Polo-López, Maria

Silva, Adrián

English

Repositório Institucional da Universidade Católica Portuguesa

52  Microbiotec’17 – Book of Abstracts Environmental Microbiology and Biotechnology O-16 - MICROBIOLOGICAL QUALITY OF TREATED WATER BY SOLAR-DRIVEN OXIDATION PROCESSES  Nuno Moreira1,2; Carlos Narciso-Da-Rocha3; Maria Polo-López4; Luisa Pastrana-Martínez1; Joaquim Faria1; Célia Manaia3; Pilar Fernández-Ibáñez5; Olga Nunes2; Adrián Silva1  1 - Laboratory of Separation and Reaction Engineering - Laboratory of Catalysis and Materials (LSRE-LCM), Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; 2 - LEPABE – Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; 3 - Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal; 4 - Plataforma Solar de Almeria – CIEMAT, P.O. Box 22, 04200 Tabernas, Almeria, Spain; 5 - Nanotechnology and Integrated BioEngineering Centre, School of Engineering, University of Ulster, Newtownabbey, Northern Ireland, BT37 0QB, United Kingdom Background  The commonly implemented wastewater treatments have a limited capacity to remove organic micropollutants, including pharmaceuticals, and human pathogens, as well as antibiotic resistant bacteria (ARB) and related genes (ARG). As a consequence, the continuous disposal of antibiotics and related products into the environment can lead to the development and proliferation of ARB, a problem that needs urgent solutions. The present study aimed at comparing the efficiency of different solar-driven oxidation processes on the removal of undesirable microorganisms, with the simultaneous elimination of organic micropollutants, in the secondary effluent of an urban wastewater treatment plant, using a solar compound parabolic collector at pilot-scale. Method  Wastewater samples were spiked with 100 μg L-1 of each selected model organic micropollutant: carbamazepine, sulfamethoxazole and diclofenac. Quantitative analysis of the organics was made by HPLC. The abundance of total and tetracycline and ciprofloxacin resistant cultivable faecal indicator bacteria and of the genes 16S rRNA, intI1, blaTEM, qnrS, sul1, blaCTX-M and vanA was followed by using the membrane filtration method and qPCR, respectively. Bacterial community was analysed based on the hypervariable V3/V4 region of 16S rRNA gene Illumina sequencing. Samples were analysed before treatment, immediately after treatment, and after 3-days of storage of treated wastewater at room temperature. Results & Conclusions  The highest efficiency on the removal of the parent model organic compounds to concentrations below the limit of detection (LOD) was observed for H2O2-assisted photocatalysis using TiO2 as photocatalyst. Tetracycline and ciprofloxacin resistant enterococci and faecal coliforms were removed to levels below the LOD (1 CFU 100 mL-1), irrespective of the treatment applied with H2O2, even upon storage of the treated wastewater for 3-days. However, reactivation assessed based on 16S rRNA and sul1 quantification was observed in all the treatments. Regardless of the type of process, wastewater treatment and storage caused notorious bacterial communities disturbances, characterized by an increase in the relative abundance of Proteobacteria (classes Beta- and Gammaproteobacteria), namely of the genera Pseudomonas, Rheinheimera, Methylotenera. References & Acknowledgments  This work was supported by Project nº P1404290052 (SFERA Program, EC/FP7-Integrating Activities), and by NORTE 2020 (AIProcMat@N2020 NORTE-01-0145-FEDER-000006; LEPABE-2-ECO-INNOVATION NORTE-01-0145-FEDER‐000005), under the Portugal 2020 Partnership Agreement, through ERDF, Projects POCI-01-0145-FEDER-006984 (UID/EQU/50020/2013) and POCI-01-0145-FEDER-006939 (UID/EQU/00511/2013), and COMPETE2020 – and by national funds (FCT, UID/Multi/50016/2013-CBQF and Water JPI/0001/2013 STARE). NFFM, LMPM and AMTS acknowledge PD/BD/114318/2016, IF/01248/2014 and IF/01501/2013, respectively. The authors acknowledge also the COST-European Cooperation in Science and Technology, to the COST Action ES1403: New and emerging challenges and opportunities in wastewater reuse (NEREUS). Microbiological quality of treated water by solar-driven oxidation processes  Keywords: Solar-driven oxidation treatment, Organic micropollutants, Human pathogens, Antibiotic resistance, Bacterial communities 

Microbiological quality of treated water by solar-driven oxidation processes

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Microbiological quality of treated water by solar-driven oxidation processes

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