Acciainoli, Karina C.

Mesquita, Raquel B. R.

Rangel, António O. S. S.

Silva, Mafalda T. S.

English

Repositório Institucional da Universidade Católica Portuguesa

P26*   MICROFLUIDIC PAPER-BASED ANALYTICAL DEVICE FOR THE DETERMINATION OF SALIVARY CALCIUM  Mafalda T. S. Silva, Karina C. Acciainoli, Raquel B. R. Mesquita*, António O. S: S. Rangel  Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital 172, 4200-374 Porto, Portugal *rmesquita@porto.ucp.pt  The determination of salivary calcium concentration may anticipate potential problems or help to improve target therapies [1,2]. In fact, the concentration of calcium in saliva can be a symptom of cystic fibrosis or medication abuse. The concentration of calcium in saliva depends mainly on the pH and the salivary flow rate. A fast, non-evasive, disposable, on-the-moment test device available to be used in a routine consultation would facilitate the overall diagnostic. Microfluidic paper-based analytical devices (µPADs) have several benefits which make them ideally suited to conduct on-location analyte determinations. While many of the current monitoring techniques require specialised skills, laborious laboratory processes, or/and expensive equipment, µPADs provide an avenue for very quickly and cheaply attain an analytical result. These PADs are composed of a hydrophilic zone, consisting of paper, and a hydrophobic zone that delimits them. The paper cellulose fibers enable liquids transportation by capillarity. In this work, a novel approach was used to the design the hydrophilic/hydrophobic areas. The developed PAD was employed to determine the salivary calcium concentration as an economical and in-loco alternative. The method was based on the reaction between calcium and o-cresolphthalein complexone, generating a coloured complex, quantified through pixel readings [3]. The consumption of both reagents and sample is rather low, about 10 L. This feature, along with the intrinsic portability of the PADs, enabled to obtain a device suitable for regular monitoring at routine consultation to be used by unskilled operators.  Acknowledgements: R.B.R. Mesquita thanks to Fundação para a Ciência e a Tecnologia (FCT, Portugal) and POCH of Fundo Social Europeu (FSE) for the grant SFRH/BDP/112032/2015. This work was supported by National Funds from FCT – Fundação para a Ciência e Tecnologia through project UID/Multi/50016/2013.  [1] Johan K.M.; Martens L. 2005, 150, 119-131.  [2] Preedy, V. (2015) Calcium: chemistry, analysis, function and effects. 1st ed., Cambridge: The Royal Society of Chemistry. [3] Andres W. Martinez; Scott T. Philips; George M. Whitesides 2010, 82, 3-10.       

Microfluidic paper-based analytical device for the determination of salivary calcium

https://repositorio.ucp.pt/bitstream/10400.14/26601/1/book-64-64.pdf

Microfluidic paper-based analytical device for the determination of salivary calcium

Abstract

Similar works

Full text

Available Versions

Repositório Institucional da Universidade Católica Portuguesa