Acciainoli, Karina C.

Mesquita, Raquel B. R.

Rangel, António O. S. S.

Silva, Mafalda T. S.

English

Repositório Institucional da Universidade Católica Portuguesa

ANALÍTICA 20189th Meeting of Division of Analytical Chemistry26-27 MARCH, PORTO-PORTUGALFFUP/ICBAS – UNIVERSITY OF PORTOBOOK OF  ABSTRACTSwww.analitica2018.eventos.chemistry.pt103 PH 15 DESIGN AND DEVELOPMENT OF A MICROFLUIDIC PAPER-BASED ANALYTICAL DEVICE FOR CALCIUM DETERMINATION IN SALIVA Karina C. Acciainoli, Mafalda T. S. Silva, Raquel B. R. Mesquita, António O. S. S.  RangelUniversidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital 172, 4200-374 Porto, Portugal rmesquita@porto.ucp.pt Saliva is an exocrine, aqueous and transparent fluid that contains calcium in its composition, which plays an important role in the balance of remineralization and demineralization of the teeth. The concentration of calcium in saliva depends mainly on the pH of this fluid and the salivary flow rate. The determination of salivary calcium concentration may anticipate potential problems or help to improve target therapies [1, 2]. Microfluidic paper-based analytical devices (PAD) are devices composed of a hydrophilic zone, consisting of paper, and a hydrophobic zone that delimits them. The papers cellulose fibers enable liquids transportation by capillarity. In this work, a PAD was developed to determine the salivary calcium concentration in an economical and in situ manner (Figure 1). The colorimetric reaction between calcium and o-cresolphthalein complexone generates a coloured product that can be quantified through the information obtained by image capture devices [3]. Figure 1: Exploded view of the PAD developed. Acknowledgements: R. B. R. Mesquita thanks to Fundação para a Ciência e a Tecnologia (FCT, Portugal) and POCH of Fundo Social Europeu (FSE) for the grant SFRH/BDP/112032/2015. This work was supported by Nationals Funds from FCT – Fundação para a Ciência e Tecnologia through project UID/Multi/50016/2013. [1] Johan, K. M.; Martens, L. Forensic Sci. Int. 2005, 150, 119-131.[2] Preedy, V. (2015) Calcium: chemistry, analysis, function and effects. 1st ed., Cambridge:The Royal Society of Chemistry.[3] Martinez, A. W.; Phillips, S. T.; Whitesides, G. M. Anal. Chem. 2010, 82, 3-10.

Design and development of a microfuidic paper-based analytical device for calcium determination in saliva

https://repositorio.ucp.pt/bitstream/10400.14/24308/1/Silva_PH15_Analitica2018.pdf

Design and development of a microfuidic paper-based analytical device for calcium determination in saliva

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