The Study of Artificial Sweetener Concentration Analysis by the Spectrophotometer

Abstract

[[abstract]]近年來人工甜味劑由於有成本低廉、甜度很高、用量極小的特點被廣泛添加在食品和飲品中，分為能產生熱量的營養型以及不產生熱量的非營養型甜味劑；低熱量的非營養型甜味劑能幫助降低熱量攝取和控制體重，不會引起人體胰島素水平，有助糖尿病慢性病患者在控制血糖的同時又能嚐到甜味，但也有研究指出過量食用部分的人工甜味劑有致癌的可能性，以及患有苯丙酮酸尿症(PAH)的患者不適合食用阿斯巴甜，因此在食用前對於人工甜味劑定性與定量的檢測便顯得非常重要。目前用來檢測人工甜味劑的方式為高效液相層析儀(High performance Liquid Chromatography, HPCL)， HPLC的缺點為設備昂貴、操作嚴格，故在本論文中使用分光光度計進行人工甜味劑定性與定量分析，分析目前台灣食物安全衛生管理法核准使用的阿斯巴甜(Aspartame)、糖精鈉鹽(Sodium Saccharin)、糖蜜素(環己基(代)磺醯胺酸鈉)(Sodium cyclamate)，藉由不同種類的物質對不同光波長吸收率強度的差異，量測不同濃度的人工甜味劑並進行樣品數據的回歸分析以及統計檢定，藉此討論分光光度計用以檢測人工甜味劑的可行性。[[abstract]]In recent years, artificial sweeteners are widely used in food and beverages because of their low cost, high sweetness and low dosage. They are classified into nutritive and non-nutritive sweeteners which can produce heat. Heat sweeteners help reduce calorie intake and weight control, do not cause human insulin levels, it also helps patients with chronic diabetes in the control of blood sugar also can taste the sweetness at the same time, but there are studies have pointed out that excessive consumption of artificial sweeteners have the risk of suffering from cancer and patients with phenylketonuria (PAH) are not suitable for consumption of aspartame, so it is important to have a qualitative and quantitative detection of artificial sweeteners before consumption. At present, the method used to detect artificial sweeteners is High Performance Liquid Chromatography (HPCL); HPLC shortcomings for the equipment is expensive, strict operation. In this paper the use of spectrophotometer for artificial sweetener qualitative and quantitative analysis of Aspartame, Sodium Saccharin, and sodium cyclamate approved by the Taiwan Food Safety and Health Administration, by different kinds of substances on the different wavelengths of light absorption intensity difference, measurement of different concentrations of artificial sweeteners and sample data regression analysis and statistical test to discuss the feasibility of spectrophotometer to detect artificial sweeteners.[[note]]碩