The role of hormones in the controlled reproduction of a few test fishes is well documented. However, information on the mechanisms of endocrine regulation of ovulation in cultivated warm-water fishes is very meagre. Hormones, especially the gonadotropic hormones of piscine origin, are increasingly being used in modern aquaculture to produce the seed of many important cultivated fishes. While chorionic gonadotropin and other exogenous mammalian hormones are used in spawning the channel catfish, fish pituitary hormones are usually needed to induce spawning in the difficult-to-spawn Asiatic carps. In mullets, however, either homoplastic pituitary gland or human chorionic gonadotropin (HCG) or a mixture of HCG and a threshold dose of the former is ordinarily injected to precipitate spawning. Of late, semi-purified salmon gonadotropin (SG-G100) has been used to induce spawning in several species of food fishes. While several marine fishes have been artificially bred by administration of hormones, induced spawning of the milkfish, C. chanos has been tried with little success. The milkfish is a widely distributed food fish extensively cultivated in ponds in Southeast Asia. Recently, significant results have been obtained in spawning mature milkfish captured from the wild by hormone injections. The experiments conducted on induced breeding of milkfish leading to the successful fertilization and hatching of milkfish eggs are briefly described
