Toksisitas Akut (Ld50) Dan Uji Gelagat Ekstrak Daun Teh Hijau (Camellia Sinensis (Linn.) Kunze) Pada Mencit

Abstract

Among many kind of drinks, tea is the most preferable drink for Indonesian people. Compare to other drinks, tea has a lot of benefit to our healthy. Tea are able to prevent some diseases and help for recover process from simple diseasessuch as influenza, to chronic hard disease; that could cause death such as cancer. Variety of tea can be selected referring to the flavor. Acute toxicity test (Lethal Dose) has been conducted of the extract of green tea leave (Camellia sinensis (Linn.), Kunze.) to the safety and symptom test as well. The test used mice as an animal experiments. The acute toxicity test use Weil, C.S (1915) method for the acute toxicity test and Paget. GE and Barnes, JM (1964) method for symptom test, high dosage under LD50 value are given to the mice, while green tea leave extract is made by percolation according to the method in Indonesian Pharmacope III. One kilogram of green tea powder produced an extract of 375 g or 37.5% of rendemen (sample). The result showed, the LD50 value of green lea leave extract was 3.303 (2.10 - 5.14) mg/10 g body weight i.p. By extrapolating the value as per oral in mice based on Gleason test (1969), the extract was classified as a Practically Non Toxic (PNT). The symptom test proved that green tea leave extract in a dosage of 2,7 mg/10 g body weight influenced the nerve system