Toxoplasma Gondii Identification in Mother\u27s Blood and Fetal Tissue with Nested PCR

Abstract

Toxoplasma gondii identification in mother’s blood and fetal tissue with nested PCRObjective: To examine the correlation between Toxoplasma gondii infection with spontaneous abortion on pregnant women based on nested PCR result from mother’s blood and fetal tissue.Methods: A prospective clinical diagnostic study using nested PCR performed on 30 cases of pregnant women with spontaneous abortion fulfilling the inclusion criteria, latex agglutination test (+) and exclusion criteria, latex agglutination test (-). Mother’s blood and fetal tissues samples, which gave positive result in serologic test, were analyzed with nested PCR using18S-rDNA gene primers.Results: Five of 30 mother’s blood samples (16.7%) and 9 of 30 fetal tissue samples (30%) gave positive PCR results. According to Fisher’s Exact test, PCR detected the presence of Toxoplasma gondii in significant value (P < .001). Conclusion: There is a strong correlation between Toxoplasma gondii infection with spontaneous abortion (P < 001)