Tartrazin is a certified colouring agent which classified into certified colour means it is permitted for foods and bevereges. . The aim this research was to determine the existence of tartrazin in orson drink. Identification was carried out using thin layer chromatography (TLC) method white mobile phase isopropanol:ammonia 25% (4:1), while the assay was using spectrophotometry method. Thin layer chromatogrphy result showed that all samples contain tartrazin. The result of analysis method that coefficient of variation the presicion was 1,230% ( r table). Limit of detection and limit of quntitation of this research was 1,251 ppm and 4,169 ppm It was found that concentration of tartrazine in samples A ; B ; C and D ; were 79,445; 77,325;78,267;78,56 respechvely. Based on the level which is producted shows that the sample which is contained tartrazine the level is not more than the standard 100 ppm
Key words: Thin Layer Chromatography, Tartrazine, Spectrophotometry, Ultraviolet- Visibl
