Spiritual intelligence is essential for nurses to be humane, to maintain a good relationship with patients, and to show a sense of caring to patients in order to manifest prosocial behavior. The searching for the meaning for the nurses is able to connect the provision of nursing services to the duty on the basis of worship to God and the help for people, patients, who need it. The aim of this study was to find out the relationship between spiritual intelligence and prosocial behavior in nurses at Rumah Sakit Islam Banjarmasin. The subjects in this study were 34 nurses at Rumah Sakit Islam Banjarmasin. Data were collected using a scale of spiritual intelligence and a scale of prosocial behavior while the data were analyzed using Pearson product moment from Karl Pearson. Before the correlation test was conducted, the normality test was performed.The results of normality test showed that the distribution of the data was normal with a significance value greater than 0.05, namely the spiritual intelligence value by 0.200 and prosocial behavior 0.200. The results of the correlation analysis on 34 subjects indicated that r = 0.575, with p = 0.000 (p < 0.05). Thus this study proved that there was a fairly strong positive relationship between spiritual intelligence variable and prosocial behavior variable in nurses at Rumah Sakit Islam Banjarmasin