Pengaruh Auksin Iba Dan Naa Terhadap Induksi Perakaran Inggu

Abstract

Roe (Ruta graveolens L.) is one of the Indonesian rare medicinal plants. An attempt to conserve roe, has been conducted through in vitro culture of sterile shoots at the laboratory of the Indonesian Spice and Medicinal Crops Research Institut (ISMCRI) for 17 years. To observe the genetic stability of culture following in vitro conservation for a long period, the collection must be tested in greenhouse and field. Therefore, it is necessary to induce rooting. The aim of the experiment was to observe the effect of IBA and NAA auxin to root induction of roe. The sterile shoots were used as material. They were planted on half-concentration (½ MS) on Murashige and Skoog (MS) medium, enriched with vitamin from group B. The experiment was arranged in a completely randomized design with five replications. Each replication consist of five bottles with two plants. The treatment tested were several concentrations of IBA and NAA (0; 0.001; 0.002; and 0.003 mg/l). The parameters observed were number, lenght, shape, and length of roots, and also the number of shoots and culture performance. The result showed that the use of ½ MS + NAA0.001 mg/l produced the highest number of roots (13.6 roots). This treatment also produced a lot of root hairs which indicates a healthy roots