: Urinary catheterization as a part of invasive procedure application poses the risk of nosocomial infection to intensive care unit patients. The probability of nosocomial infection increases in accordance to the period of catheterization; longer catheterization time yields higher chances for microbial contamination on urinary catheter as catheterization provides an opening for microbial invasion into the urinary track. There are various pathogens commonly associated with catheterization including Escherichia coli, Klebsiella pneumonia, Proteus mirabili, Pseudomonas aeruginosa, Enterobacter, Staphylococcus aureus, Enterococcus fecalis, Serratia and Candida. The main objective of this research project is to observe bacterial pattern of urine from catheterized intensive care unit patients in the Prof. DR. R. D. Kandou Public Hospital Manado. A total of 20 urine samples were obtained, one sample from each individual catheter over 2 months period. Each collected sample is subsequently passed on to microbiology laboratory to undergo bacteria identification process via culture media. The preliminary results show that the identified bacterial pattern is Staphylococcus aureus, Candida, Streptococcus, Diplococcus, Proteus vulgaris, Lactobacillus, Bacillus subtilis, Enterobacter aglomerans, Citrobacter freundii dan Shigella. Following an in-depth multi-dimensional analysis of preliminary research results, the conclusion can be drawn that the most commonly found bacteria is Staphylococcus aureus. This study is part of growing body of microbiology research in catheter-related bacterial pattern and nosocomial infection; this research project will contribute to future research on similar topics