Accurately assessing the ageing status of oil-paper insulation in transformer is essential and important. Polarization and Depolarization Current (PDC) technique is effective in assessing the condition of oil-paper insulation system. Though the PDC behaviour of mineral oil-paper insulation has been widely investigated, there is no report about how to make the quantitative analysis of mineral oil-paper insulation ageing condition by PDC. The PDC characteristics of mineral oil-paper insulation samples were investigated over the ageing period at 110°C. A new method for assessing the ageing condition of mineral oil-paper insulation by calculating the depolarization charge quantity was proposed. Results show that the depolarization charge quantity of mineral oil-paper insulation sample is very sensitive to its ageing condition. The stable depolarization charge quantity could be used to predict the ageing condition of mineral oil-paper insulation