Aarestrup

Del Villar-Guerra

Dieperink

Frier

From

Geertz-Hansen

Hansen

Jensen

Jepsen

Koed

Larsen

Lorenzen

Mortensen

Nielsen

Otterstrøm

Pedersen

Poulsen

Rasmussen

Taastrøm

Thomsen

Ursin

BMC Infectious Diseases

Valérie Chaignat

Marina Djordjevic-Spasic

Anke Ruettger

Peter Otto

Diana Klimpel

Wolfgang Müller

Konrad Sachse

George Araj

Roland Diller

Herbert Tomaso

MUCC (Crossref)

Performance of seven serological assays for diagnosing tularemia

Springer - Publisher Connector

Abstract
          
            Background
            Tularemia is a rare zoonotic disease caused by the Gram-negative bacterium Francisella tularensis. Serology is frequently the preferred diagnostic approach, because the pathogen is highly infectious and difficult to cultivate. The aim of this retrospective study was to determine the diagnostic accuracy of tularemia specific tests.
          
          
            Methods
            The Serazym®Anti-Francisella tularensis ELISA, Serion ELISA classic Francisella tularensis IgG/IgM, an in-house ELISA, the VIRapid® Tularemia immunochromatographic test, an in-house antigen microarray, and a Western Blot (WB) assay were evaluated. The diagnosis tularemia was established using a standard micro-agglutination assay. In total, 135 sera from a series of 110 consecutive tularemia patients were tested.
          
          
            Results
            The diagnostic sensitivity and diagnostic specificity of the tests were VIRapid (97.0% and 84.0%), Serion IgG (96.3% and 96.8%), Serion IgM (94.8% and 96.8%), Serazym (97.0% and 91.5%), in-house ELISA (95.6% and 76.6%), WB (93.3% and 83.0%), microarray (91.1% and 97.9%).
          
          
            Conclusions
            The diagnostic value of the commercial assays was proven, because the diagnostic accuracy was &gt;90%. The diagnostic sensitivity of the in-house ELISA and the WB were acceptable, but the diagnostic accuracy was &lt;90%. Interestingly, the antigen microarray test was very specific and had a very good positive predictive value.
          </jats:sec

Crossref

Rasmussen, Gorm Heilskov

Pedersen, Stig

Online Research Database In Technology

Sea trout (Salmo trutta L.) in Denmark

file:///data/remote/core/dit/data/Springer-OA/pdf/410/aHR0cDovL2xpbmsuc3ByaW5nZXIuY29tLzEwLjExODYvMTQ3MS0yMzM0LTE0LTIzNC5wZGY=.pdf

Performance of seven serological assays for diagnosing tularemia

Abstract

Similar works

Full text

Available Versions

MUCC (Crossref)

Springer - Publisher Connector

MUCC (Crossref)

Crossref

MUCC (Crossref)

MUCC (Crossref)

MUCC (Crossref)

MUCC (Crossref)

Online Research Database In Technology

MUCC (Crossref)