Arabi Monfared, Ali

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,{tMaL)1{i(/'00,,,rS*},;4 o6,i,ll.l,rS,i,;4 ,r.,*,1'..1. 6;G <i,-x.; .r,.},;l .,-,-l'.,1,-rL( &!- *L .rt*!:;13"c;f+; -l LE csSJ. c*.;G ;; -il J*fn -l cit*$y .r*LG.l;ir LCJc .,.L ,t r.l,.r!ry,,l*-K;iS.r.6.1*.,e .,"ell .ti Cr1.l r.r"'riS.l ;l ^"4t3 .rE-l\Y11-\Y1o :.J*--: J1*,Kerman Universify of Medical SciencesFaculty of MedicineIn Partial Fulfillment of the Requirements for the Degree(MSc)Title:Determination of chemical composition and anti candida activities ofMentha spicato and Trachyspermum ammiAromatic watersBy:Ali Arabi MonfaredSupervisors:L- Dr.Seyed Amin Ayatollahi Mosavi2- Dr.Kamiar ZomorodianYear:2017./u*'rr",;.-,(/"4,oQ"' ln Y;-{til)D+5'dpl .,- .J 6L*;l p v tt ,- -.p .*rii .S,,L* cr-r eb 9 ar-r*ed jl ,!- o--jt-"r,-stJ :r-!t.ral9 as.r.Lo'-tjt 5 - # Dlt r-rl+ cl:.15.rt" qU b A+.1:x +-U.xf ,s^L- ;\ Ur 9 .rt' co.; ,sl> c,s9,.o { cs,rLr:c;9ie9.t- ,ri ,rL-l 9 r*U;*.jtak; G,* )rbdt rriti,r.,J-.SI-J'[l4.r;tf ,;.stL*,-pb ir,;o4d-L or-.t Jb*- L-lr !,.., t{ ct r-itr.r yb ,z-rs Jjy\J.'- &g116,-1.c.-l .*.rE.rb;L.r .sloq a+t !..r,F Sr G-;,L1:,[,ti f 9 ef cjta cirre:[2xll o+\e f,p )lq-_l ..:sL ,.r:^,-l i* ,4.5l-o91ll 6L;: c-.:.; 69*^,-19;l .rl-,1*r .drLr- .j' ,-l d.+ dr .r--,_l p:*.- .FJ .51.,1: ;lrl"o ,L; ,:11;sl,L-bF ,s{tl-Srrr ylr+ Jllir 'l 9 e9 orLo;l q p 9 s,"*+jv:I6,-1,r,-91r; &16,rqr,irr ,;^'-r ,fl9ryjl..-i-.'- ',l uiu "ri,-lFl ;)n-llr^*ak: rlr,,) e-.,lqr jl 6:e*qe i,,taf 4-1-r Jr,u,-9srsdp-ld te"i;l.r-itr .r.rbL*f ot-.if )9j .r 551;:".1 [o ,y:S ssjlX: jl aS u,-9]s,ra[f o[f; Jt<,:lalf- o:,lrJ ol,,:f ,sai:;a r.r";. c,UlL^ r: to ;Ll ,:;9fu ,rj c,l)ilf S! .r olof ,n;Ll .Sft c,ljf;,*o; a*lLl- J--l .i"ra ;u;! .f o:Li;l ,!1i .frl* ..lL,r) ,1: [.o911.:;L lr atj* otf 4.L- .51il> .5911:.ulr.r 6 ctsi 9 d ott L-|y,9: p l.r,-stf .5lop; qJr tb t JV).+tf ol.!f .;rti "r; ol;l)rr.*S olf ,r-l:;;V L-"; o_f el;; 9 OV) eV uf g: citj)o;la*llt ;_l.1: :ej9.p 9 st9n,il,-Llrl.r) Jl-l> 1lo.:lallr olif f )*9n ot{,;.r_LL-i, ,9t;,- a'...r6 o:li; ul eF ,lf .q1b 9 .r,_Ll:;vL5 jlo.:l;r,.1 lr ,-n;LlaJ(* ,11;l,GC/IVIS ot(:-.: *,g-tit{ "rit-l&u;, f t 4)F l.s oljlc;Ll:r9.- .Wiley ;Lclltl a-;b.l5rr o I .i )-;;, .5Loo.rl.r t-., yJ, rr cst't &*fu a*,-l-i,9 (Adams, 2001)\6 ,syy. tb r Otr; c,l.!f (MFC) ;r)# e (MIC) ;i;,, ,,\e ,rir., il a,JlL .1,1 p .uf ,lf .,,_Lt:j.lLl,-r ;;et-ber<e ol* Gy u. 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AV) Gf .)r.il ,rr l" of Fl yV* tV o* trJ+r, Jt( :'i rl-aa e.=L tL; ,.1,.s , \/f9 \/\t c-i, P 4i 4-{ r-r) 1' 9 r-p a'OlF a; (10261.r,-),5 \/\i c.lrrr "lals A. a1,,.* q ,r,-1.r,-sll.rl+r.p oL+-j bu ;v jlob Af .r--11-"r,-st-f ,.rL.r) e JF :t aV) of 6r;'. il:tgf $.f,;L! .:rj d-btL "[t! olr:1.:.11: 6rs O:l t-f "jrF .st" c^i! r".-.,, * "(J9 ,]&, ,_*rtl A*lli#.'7.rL! , .' .rl* n,tlfl.Str.;f t) )rlf .b Uiyl" olJf .5UU cJi.lc,:.[q a, sly .f AV) Ofst o)es.*.rL" c-i! ei tusx ,,Sf f )f f *V ;.il{y'l "r; e ;l;r,Sl ei 'elf #, q cE}tL '[1t!.r2l: .5ldLllL.-!E "alt JiS l. a^..*ti..1: dllif ! o.r.i;L1: ,sl" ,-FyJltJl .,.,i,,"lix ,tV; Gf ,,;,V) Gf ,lr,-sl5:dr*15 "5tl ojlgAbstract:Background and Objectives: Candidiasis is one of the most common opportunistic fungaldiseases in human. Candida species which is considered as a part of normal flora ofmucocutaneous surfaces, many cause wide range of clinical symptom from superficialinfection to mucocutaneous or visceral candidiasis. These yeasts may also cause uppergastrointestinal especially among those with imbalance normal flora or compromised immunesystem. Because of the universal increase in antifungal-resistant Candida, there is growinginterest in furding novel antifungal agents especially from natural resources. Among which,aromatic waters distilled from medicinal plant containing essential oils with knownantimicrobial properties. The aim of this study was to determined in vitro antifungal activityof Trachyspermum ammi and Menta Spicata AWs and in vivo antifungal activity ofTrachyspermum ammi AW against Candida species.Methods: The chemical composition of the essential oil from T.ammi and M.Spicata AWsanalyzed by gas chromatography-mass spectrometry (GC-MS). The components of theessential oils were identified by comparing their kovats indices and mass spectrafragmentation patterns with those stored on the MS-data bank (Adams, 2001 and the Wileylibrary).The antimicrobial activity of the essential oil against Candida species was evaluatedby broth micro-dilution as per the Clinical and Laboratory Standards Institute (CLSI)methods. Moreover, biofihn formation inhibition and antioxidant activity of the AW wasmeasured by using a XTT reduction and DPPH methods, respectively. Experimental activityof the AW in the prevention or treatment of GI candidiasis was also evaluated in animalmodel by both culture and histopathological methods.Results: GC-MS analysis revealed that the major constituents of the essential oil of T.ammiAW were Thymol (78.08yo), Carvacrol (8.20%) and Carvotanacetone (6.50%) and Carvonewith 55.97Yo was the main component of M.Spicata aw essential oil and Piperiton and Di -hydro carveol with respectively concentrations 15.91% and 5.77o/o were ranked. The T.ammiAW exhibited antimicrobial activity against all tested yeasts with MICs in the range of 0.125-0.25 YN.In addition, the EO inhibited the biofilrn formation of Candida albicans atconcentration up to 0.25 VIV(90%). M.Spicata aw almost the most species was ineffectiveand was inhibited 50 % biofilm formation in dilution of 0.0625. The T.ammi AWsignificantly decreased the CFUs in mice receiving AW in comparison with those of controlgroup. Similarly, histopathological analyses showed that candidal colonization decline inmice following administration of AW of T.ammi in therapeutic trial.Conculation: The considerable antifungal activity of the AW against the examined Candidaspecies might be related to high concentration of phenolic monoterpens in the EO distilledfrom AW. In addition to considerable antimicrobial effects of the AW, antioxidant activityactivity of the AW attributed to inducing the healing process of tissue necrosis found in micetreated with AW in comparison to the controls. Considering wide range of antifungalactivities of the examined AW, it might have potential to be used in the management ofalimentary Candidiasis or as mouthwash or other pharmaceutical products.Key words: Candida spp, Trachyspermum ammi, Mento Spicata, Aromatic Water, Antifungalactivity.

شناسایی ترکیبات و تعیین اثر ضد قارچی عرق های نعناع و زنیان

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