Monirzadeh, Fatemeh

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,rtff,(,t",rft;1 o.lS..i,it.r,,S,.i3,4,r*, l'.,1 *OJ*.ai.iJ.r,.irl .r-t'..+,:lS n:r. $t*:l,'.e i q-E rtnknlr,:.r#J# ., cr.s el-rigb u*oS:- J; il s cs+l*J# &l1$i ! c'i-l"'- n**r1y.r-r* )tj*,J( 69t-a d3;Xl ciJJ ;,1 copA 6i 3 cepA s qacEll csL^ ,i JJr?31l-toJL* -r.r nL;S )4.X 6 t, ,rL.rL:* 3l ori orrl 65otl-;t+ d"tl-i : L. ...-f.,*t11(,.}, L iI .L"-- ;*Sl :t-r"al, .ll':' "l\Y11-\Y1V ,J*,-=: Jl-eKerman University of Medical SciencesFaculty of MedicineIn Partial Futrfillment of the Requirements for the Degree Master of Science inMedical IVlicrobiologyTitle:Evaluation antimicrobial activity of two biocide compunds, a naonocoppercep,A and copA genes on Klebasiella pneumoniae and study af qacEl1isolates collected from Kerman hospitals during 2015-2016By: Fatemeh MonirzadehSupervisor: Dr" Mohammmad Reza ShakibaieYear: 20171[t4(,pltit"J?x.#-rG o,r$+"j+- ,uftl-,,'*: iSlo ,lf.X .r) us*&, "Jfi i^lt *P J'ji\.!. 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Lrla iit'tLjiI:*, gl9;i .c-i or"i Lir .r-b,r- rr ur+ .,jl,6 &- lh"l e-oL Lt+"X c'V y q tutb i,,*+.n : 1",i, ,+,r";l.p orG;l .:19, .;p* +-l b ujt!. tfu * ,$X flf I b ".ri;.r{.*l ;r tai;;.l.Ji I i+- ;i :19. ;tL.g+.-9.{ c,qif q ,:-lb u.ui sl-:,-l "i"it of qacEl } 9 cepA 6la $ Ly!. *:y. e-"-; ;-l ;i jro .:p<"l-,"8 ,t ry:rl -LJr * $y+ . \-.1t *!lq .5i" aj9ll c-1,- ;lr" .;;:o ,'-l 7: ;l ;' o9){-o '"*F "tlf ts.,s.,4 )ty srfu 1l;! o":h**i ,tf ..,bll** :t Gruo# c^q7 af "&f, I r,-l,Jt e:ot(lfb6 ; n ;,9U. li-71i jr:,* aS copA j.; L9r., , ,- olrrg;l; a, .3*ii:a' ;l 7r a,JtL rt," .J[t aJ9;l c,*-L-.>.4.;f ,.:-:"* op o159rL 1L5;.: "5.r,L 9* 66 l,rJ-"9x1*i ll --W lr .$,5 )lF aJlt" :19o r:ul ,u.St" Oj )p I "* g.rsle.: jL$tt J ;.oq ,U,.i ,Lrui dta .rbrL* :l dskl AA rls :b Oi9, g llg", (utC) r;r il u5r:Jri{" c,.ltE U-;*S 9 Lsf tlp ,t"ry r19" PCR' "(rti L copAg qacEZl,cepA,l^Ji,9;r o"fil r-* c,lri9;b 9 ud.9f t,+,r,f r:*tflip .i,t..Jy (MBC) l;r;l .r5r;5 .-tle .r,iJ.loix J*(.r d,*q,*^.o.$f e$loi; ;;rtbrr(-.y:, q (C[.SI )*!!: *oti:-"u;i.5torrisb-la.*y'.-r,5 rEjl y*sf* ,-*s, * orii o;!9:b 9 rJ.:9fr..r-f r5,1t(Jip nryi *^iix # il s3jgc;ir:99(r-1r1\,A)ai,.iYlt,tcepA u_,.ir(-"r.f.).r-Yf, pcopA,,jdti-lAdab;l :61tirf #* SY .s ojL r) r-,Jf FrrKJ,ft Lx;t', e,U.tt u512, MIC "r,-)F oroL;j-" 5l dei.l 49 ,t qacEA ld9;l r gi:,;l aJ9;i Y. ,L" ;l 9 s:l; ;k; .;9Ui, d* o1!;tj a, 'o ajr.p-l pL; 9 -i,-":r-f JtF A u,*" ]Jbl- ,'lix 4ll.j Y ,J"*f: ,t-er '"li* dltl c- ra .{l/5 pr#t(ll .A *, i ,slx.srlr ,-f,5-::' esf r'l-ix"tlS ,[r5*r -y'"9*.t {:- \ S,.:";; oli99 zu9 ; 1 t-9::" .li9+r df-\ ozii; 0159t; ,.q; roliJ* df 1 fJpx h d9;l ;_l;l .i" er",,) *jt' ,"1#*: ir 6i-a a)ey.l 6;..5 ip ,.er f )l .r-,L9.-r,-rf; oroL;.g2;)"r9, u- o2i9;b *!)tiy i,-y'5 p9.,t(ip 9 "r"if) c,l*fr af rl:;L.l:-l J*iri"J,-leLJ *lt 1fu:rgfi aaa;;rSio Oj *e ty| "rl d.il,, dp7..o ,.u.iL .r, .r..b ol:,5*. r; c*L' .5111: .iii:.; ;yl 1r orU;io;:9;U 9 q,*t frytii;i l."J:f, c,Ul S.$-rF or-a'uia V! -t*Jy qc-9L gcapA g7acEAl,cepA."rj.:l: 6L;;-; rr"L. ,Ai9- * il S*,"+9x1 af 6p oroLL f.rc ! a*l ! "r.^.r, l;S p9-;t<l1x,u.*f .,q ac EA I g),c epA ;j,c opA oi,4 y y1 \*Jt :dJ*15 gtol5LP.{}:rlt";,rct.Introduction: Klebsiella pneumoniae as an opportunistic pathogen plays an important role inthe presence of nosocomial pneumonia. septicemia, urir-lary tract infections, wound infections,iCU infections and neonatal septicemia, and infection The bacteria are often associated rvithbiofiim production. Due to the emergence of multidrug-resistant Klebsiella pneumania isolatesas well as the resistance to biocides, it has caused serious soncern throughout theworld. Different types of antiseptics and detergents are used in hospitals and medical centers tofight this infection" The purpose of this study was to investigate the prevalence of cepA andqacEll genes in the resistance to biosciences and also to assess the sensitivity of clinicalisolates of Klebsiella pneumoniae to quantaray amrnonium compounds such as benzaikoniumchloride and deconex as used disinfectant ir"r irospital, trt also investigates their sensitivit5, tocopper nanoparticles and the prevalence of cr,;p,A gene respousible for the resistance to coppercompounds, as well as the study of the antibiofilm effects of these eompounds witli silvernanopamicles"Materials and Methods: 85 isolates were collected frorn A^fzali, Shafa, Bahonar and Kashanihospitals and the presence of cepA, qacEl I and cop,4 genes was investigated by PCR technique"with the h4inimum Inhibitory eoncentration (MlC) and the Minimum bactericidal concentration(MBC) Benzalkonium chloride, deconox. and copper nanoparticies were prepared according tothe instructions of, the Institute for Clinical and {-aboratory Standards (CLSI) by brothmicrodilution" Also biofilm and anti-biofrlm effect of benzalkonium chloride" deeonox andsilver nanoparticles were performed by microtiter method.Results: Among 85 isoXates * capA gene 34 samples t4A%) and cepA gene were present in 78samples (91"8%) and the qacEAl gene was not observed in any isolate. MIC for deconox andbenzalkoniurn chloride compounds was reported in the range of < 2pg / ml and all isolates rvereresistant copper nanoparticles. Out of the 20 seleetive isolates" three isolates were fbnnedhigh biofilm . For benzalkonium chloride, allthree samples were rnoderate biofilm , deconnex2 samples have flormed mediun'l biofrlm and 1 sarnple have low biofilrn" silven nanoparticleisample have medium biofilm ,lsample have weak biofilm, and n sample did not forme anybiofilm" No significant biofrhn was observed.Conclusion: Generally, the results of this study showed that deconox and benzalkoniumchloride eompounds have an appropriate antirnicrobial properties, in contrast to the ooppernanoparticles used in this study. there is not relationship between the presence af eepA genes,qacEll and copA and resistance to these compounds. deconox and Benzalcanium chloride andsilver nanoparticles were shown to have an adequate biofiim effect riue to the absence of anystrong biofrlm"Keywords: klebsiella pneumonia, copA gene, cepA gene, qacEllgene, deconex, benzalkoniurnchloride

تعیین حساسیت به ترکیبات بیوسایدی و اثر ضد میکروبی نانو ذرات مس و بررسی وجود ژن های qacE∆ I و cepA بر روی ایزوله های کلبسیلا پنومونیه جمع آوری شده از بیمارستان های شهر کرمان در سال ۹۵-۹۴

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