Beigi, Shirin

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Atf c/Ltr ,-*lr+ ,.:L-p : ,#X t_rtr.l5;lrd;tls lk : ,531.*,.311'; o.t(^Lils63Ly.1l: dye 6l-Ft uU.lt_t,: bli,e,Fp LF : $*) oJ^i r-i)t*:-l ,.:Li*, ,5:l.t ; Jj*'c,f*^, Lf)t,1,^5+9 J* 6La o.:-1 rSJ) J. &-il.tr,-l "[.:.-Y-JjrLo #l ,-#_* :HT-29 r MCF-7tbt,& ir.,.J*-i:61^"."! qtstt* dV -Fto$-* q4 Jt"'-l F')$ u,tu| * -5,rW1\A:uti rl!L, .;L*^,, \Y11 oL .:1.:-;.Kerman University of Medical SciencesFaculty of PharmacyPharm. D ThesisTitle:Evaluation of cytotoxicity of (Flourine and Chlorinesubstituted) Phenoxy and Naphthoxy ethyl S-nitro 2-methyl imidazole derivatives on 4549, MCF-7 and, HT-29 celllinesBy:Shirin BeigiSupervisors:Dr. Saleheh SabouriDr. Ehsan FaghihmirzaeiDr.Ali AsadipourJtul 2017 Thesis No. :918- 'G oJl--a)r-i.Ji{z..t:.(.. yt " 1-, g:L-j :lil aJLT +5 c-*1 jlb:- cr-,tti .>)t<j- C-W )l 6_ o:Jt'l: ,uJL);)-al rrL-:r LJ.JJ.J::-"-a iJ-,1"*;1 oL;: tsl-t e.): "*! J!, ! jL+^-a jGi-.-" -rl;Qt d,l-/ F-)l-r ,GVt* tao uzl7,J*U ,f .r" o:[iil aVf ,tL;: 61, o-)J)"\6 ,_r-"tr3 +;:l: e-r- St J4 JL,; ..jb JIL q a--LL i Gb_r- dt^ JIL .r-l! .," .,,L;: "r-=:drLi:-;- .:rl: :3-z-J .,-J-*rb I*"t;' ,Jlbt-. e;l--a ,:.iti -r.: .ri-,1: SF-,. ,-s;tf * :V4--i ::: i JtiJl r#,;*L- il,'-l _,:l.tl.1 i:-* -s_t" b-l;;: -1-r;lJ"--l:;-,^:K ^a\ \ *y ! .;u-^, ./ oL:j -1, ;Lr: ,_:.:- ejb-r-rl: a, l-*l 6-;*5 aQ q,o:\1"gj o); a+ 6J) f. J3;1,-t-.,-13; .-'tii-;l o-f-; ,L cS[_^y;l.r,-il 4-Jt-t" .-r*1 ;> ,-t-;.4; :l; ,r:t:;y MCF7,HT29 ,A549otf .g;L9;1: o-t-(;ll, ..-,lF^:; t-r; o-u alL;l r,, Jy;l-L-,-lj; .-,tc*; :16 ,1i-tlMTT Proliferation Assay ds,s 4: L-^ j1 ;-r"--,S i y-".:ljl ;*-' .4; ,1_p f ;L-l ;.r;: 3 J-; orlr i5 4jL; q1 -rl .futa f :) J>L ;lf \ . rlr*J 6 o;yc;-L :r.:; ft+ij--*::t31s 4;3;\:9 or: 1::- 6s:6 Ot::".jl:r=. Cl&)i ti UJL,-u-;.r^:.;tKJ;G.* ;: J;S Sl-+:f ,_r.:L-,i )J) l;: .rl-Lj;L; ,-.rt- YL .:-r-, J 4 J;:S a15"" ^.J J--r-S d;L^al UIL- a- MTT e'* rl ,;[";,l l-;.-cL f f. ,gtapt; )) cr?-.-t ^i,Sy-.:LL ,r-\-)sr.5k=bJ-",*^"i !,-rL-L-_r).-ti o$lJ, *sL i _,1 *1.t.>r^.lJ- OIJ#jl_ i J- \ - t.J--r_$ r*t--." j;1.: p .5lr- (ICso) kJlL- l;; ;r 6),o)) 0. _,k i-^lti 6 j;\: ;\ 6;js..Jf gta.;l SPSS 6;G\ ,tyt f; jl "rLi.il L y Probit ,ui t, IC56 t*L*."4549 ,HT29 ,)tJ o): tSJs, GCso;1r.5) t\ ..t--r*,0-u: E[+Jl .iq-G." -Ll ;:6kJ44 A;t) r-, BNP :-5, 6ly ,*\ i*-L IC56 glj*. . -uiq." BNP ,-J; ,. L-r._t MCFT J, \.Y .. ^;: a,4FP -5t St; ,; ,j-," ; f F:f* li rq-\:li :-,r .._)* e( 6)_.,*, lF tr*qo r \rt y \ \ \,--^--;; +-.4CP y-Sr,slt : A )* j f; rf-- Ao _q \Yt)=" t r5r,Q \.13 \.vJ \ft t-yl !PHN --"f;.gl;o-r; r-t-."IC56..:;, ;) *9 - /.\/ Jl - .-' - J vJ. t .rr,*J|* o)-, a-a .S1; ,#-1, t-.; .St^ c-tl; ! .-u c-*j -Ji 61 ;t :^5:, ;tg :6f ,aa;slr: t4 .,|-. ;: f _$:fw \ . . )l r:J ICso ,51;1: +5 :y BNP ,?=Sl dr*..-uul; c:*t_y J-*- o)J a*. -:MTT ,JtS c:; cJyjl-L--l _i"--Y-r:* -a ,J)- i.r* ,d ist:6+15 ;L*,<,.>trpAbstractIntroduction: Nowadays, the most important problem of the world is cancer, whichaffects many people each year. Therefore researchers are looking for a cure for thisdisease. The methods used today to treat cancer are surgery, radiotherapy andchemotherapy. The growth rate of cancer cells is more rapid than normal cells. Tumorshave hypoxic conditions. Nitroimidazole derivatives acts at hypoxic conditions so theyshow more specific cytotoxicity and less side effects as compared to commonchemotherapy agent. Based on this, the cytotoxic effects of new nitroimidazolederivatives were evaluated on three cell lines, MCF7,HT29, and A549.Method: The cytotoxic effects of derivatives of nitroimidazole were determined usingMTT Proliferation Assay as follows: 10000 cells were cultured in each well of a 96-well microplate. In the logarithmic phase of cell growth, cells treated with differentconcentrations of derivatives of nitroimidazole and Doxorubicin (as the positivecontrol). In each stage of experiment, control groups of the vehicle were alsoconsidered. After twenty-four hours, MTT stock solution was added to the cells, andchanges in adsorption of each well were checked after 4 hours incubation in the end,dose-response curves were drawn and IC5e values were measured for each compoundusing SPSS statistics software.Results: According to the calculations, the best effect (the lowest IC56) on the HT29,A549 and MCFT cell lines is related to the BNP. The ICso values for the BNPcompound on HT29, A549 and MCFT cell lines were 94, 96 and 64 pg I ml,respectively. The values for 4FP were 102, 129 arrd 85 pg / ml, and for 4CP were 111and 134 and 95 pg / ml. IC56 values for PHN were calculated as 134 , 107 and 106 pg /ml.Conclusion: Four out of five compounds were cytotoxic for 3 cell lines in relativelylow concentrations. The best one was BNP, which had an IC5s of less than 100 pg / mlfor the three cell lines.Keywords:in vitro, cytotoxicity, 5- niffo-2-methyl imidazole, cell line, MTTII

Evaluation of cytotoxicity of ( Flourine and chlorine substituted ) phenoxy and naphthoxy ethyl ۵- nitro ۲- methyl imidazole derivatives on A۵۴۹ , MCF-۷ and HT- ۲۹ cell lines

Evaluation of cytotoxicity of ( Flourine and chlorine substituted ) phenoxy and naphthoxy ethyl ۵- nitro ۲- methyl imidazole derivatives on A۵۴۹ , MCF-۷ and HT- ۲۹ cell lines

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