Beigi, Sheida

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,(P,r*,e,.\r/ ,, _/ ,v ,Z,,/tZ .2  /',,{;.-k6..oJL.-s cy'brr ,-#l+. c.r[..ri t '#e 1* ot^fuit,G-ttlt 1* t e)Vt;ls o't'#:lr6)t-31ls e-* Ol-f , ""U .lt-t,: t\;-e9 L,. c.*rJ," -2) oS -ey*:-l crtl5i ;l ,grl*.; J!*'c -,,4-d rf )t.gt-ai or.1 6il -t Jyjl-t *,1 J*-Y- J-#-0 ,Yl ,r.S -r,3 ,,P r.Sl.rt,HT-29 r MCF-7 A549 ;rL,:biAI;;t:,r1LJll.; +til-F aJV f :,eli-,* qC !L""-l -f t)e LsJ-*l ,* "f tt \ tr:*"tj ,)tt, o.,Lo,i \fq1 oL" rl:rrKerman Universify of Medical SciencesFaculty of PharmacyPharm. D ThesisTitle:Evaluation of Cytotoxicity of metu and pilru Phenylsubstituted, Phenoxy ethyl 5-nitro-2-methyl imid azoleon A549n MCF-7 and,IJT-zg cell linesBy:Sheida BeigiSupervisors:Dr. Salehe SabouriI)r. Ehsan Faghih-mirzaeiDr Ali AsadipourJul 2017 Thesis No. z 9196-)\i, or<-?or5-?f--a jJ- g:Ltt ,rl .:*l *-S O:-r* ob-r a^*j :: e5 ,Srt-j 1-9L^ $r+ C* :u.tir3 y ,!V ,yp GJf - * (.,Ln (J;,,Ltr jl ,-*: J o)Jl.i ti: ,"f*:,/ &y<j- c.-6:t jl ,-',g-,t^* ;.1 oL;.: : e# , 6rQ d*s -s) -rU-,c; a-fi ;)x^-a ! -t;q ./ Jq- )) f tJb'* oL;.> .51r, j.s-- .-5Lo .,,"y-, .c*1 c:r5 t+ ; ,g1 ,:;3 ,t'il o-<! :;l: +"1:l kjj tua-il- .-,Li:-;" .:r5 o;L;l C,l-t-*.>\: S .sol_f ,.r-iL;r .^-;4i jlJi .s-" 43 rrl-: r;rJ1iJ. -u:-"-o ,s-)1. (+: ;> olbr c.tL-r> .-i-ta Gl, a5 -*-"-o ;V j dL? jl ,J_e;1-u--l3r-!o-t-i _...:*g J-i;^. af\ L.i iF ^S J3,rlr*-ly; ,q-q .:U:; ,>\yl 6r--:-t q nJU" ..r-1 -r:3 r-,Ll:^:-. (,5-,:"i -tj .JiJq) 6ik sJ ,-'l;l +^--U. s .Sbr JIL 6Lr o:; -* e s; t.)t- el a:il:7 LA .-iy*-l.rtt'6;L3;l-: o-t-(:-Jlr glr-<-:-a3i -h-r:;-u: +:--L;l -t1 J3-,1-u*-l3r;.:tc^; rS a,"-l-tMTT Prolif'eration Assay ers_;4- L^ ii .r"*-Siy.-.rljl -r::-' .;-J; rl-p L;L=l ;:;; 9 ,r.-; o:1; c--5 96well *,.b .fuq ,ra ;) J-rL ;1r, o) )lrn' oS a_tyz "e{ :-u: pia.,i.r-*--1,:tSJ) GJ)\) 3 o-Lj ,* 6t-s:1).:t-c*; -i-L; 6\4*Ji ti UIL ,J); u,;.-;L(;1,.$;r J# S$:f .r:t-,1 :s);^j).J-;r:;L-;-"L Yi. ;l*; c.-,:" J,5 o1,;-" o,9Jr .-,1,*; s x-r_$ 4!tjl L€JIL a, MTT _,1.j jl -r,- c-^cL Yi ,5tgt; )) .-r-i- .-t ^*;3;1r;1 tS;s> ,e>Liu,F!-jyr.5ta^:--"" f.-r, q oL-L- -,) .-ti.IlJ, c-.eL i -,1 -tt u'ell -.[-lC.io ...-.,r.*, .)-t; a^ *1,>.n i-,1: ro gt, (IC:o) kJ+ L.:;-: G-ro-s> 0' .1+o "-;L,j.;);1t+,1 SPSS g;L1 ;l;-il fl jl .:tr;l U 3 Probit --II.r")li.+<a4549,HT29 ,)* o)) 6J); (IC5e U;*S) y\ A-:*,oJ; pL=l o!-t-." -Ll ,:6ki7F:F* 1q J VV 3 \ 'A y*; a, o-[,i c--L IC50 Jlj*" )Jr.3,4C ?5,j y Ls:y MCFT J.=.1ts:f- Y" ;-j o); a--u f tSl-t * r1-, \"5, j:IC56 "S q-51. r::lr:" -l )*,t\14 J \ 0A y+J, a"HT29 JtL- o)) ,SJ) /.3C4F J 4C3M oL5;, IC5s ,11;*. .t-y ,) *!:.1ir, !MCF7 6r) j: A _)* -t 7-s:5* \y\ 3 \tq u jtj.A549 -il .v -r. r-S:f*.r q, ! )n -r r-S:f* \ \' r \AvJrJ o); 4+ 6J) )a.s:^"L. ,)rL c?*- .:lyl .gl;l: o-r..: d*.j =.5; 4* )A :6$ o4or; y) S\-e ;4 .r!" -,.: f -S :t \ . , jl -.5 IC50 61;lr e5 ) j. 3,4C ,;15,j ;l__;e .I:_r)),I1T29 gl; \ . . ,l ,:^:r; uS : MCFT J A549 ,)_)-MTT ,.'JJL o:; ,Jyjl-t*l ,,-J-+- c^# 'd js:,.:.5+5 crL.i5_^-J\J lo-6*IIAbstractIntroduction: Despite the advances in the field of cancer, the disease is one of thelargest medical problems after cardiovascular diseases, it is the second leading cause ofdeath in the world. Therefore, researches in the field of cancer prevention, diagnosis andtreatment not only continues but also has found wider dimensions. One class ofcompounds which have been investigated for cancer target therapy is nitroimidazolederivatives. Therefore, in this study the cytotoxic effects of some nitroimidazolederivatives synthesised previousely are evaluated on cancer cell lines.Methods: The cytotoxic effects of derivatives of nitroimidazole were determined usingMTT Proliferation Assay as follows: 10000 cells were cultured in each well of a 96-well microplate. In the logarithmic phase of cell growth, cells treat with differentconcentrations of nitroimidazole derivatives and Doxorubicin as the positive control. Ineach step of experiment, control groups of cell and solvent were considered. Twenty-four h after treatment, MTT stock solution was added to the cells, and changes inadsorption of each well were checked after 4 h. In the end, dose-response curves weredrawn and IC56 values were measured using SPSS statistics software.Results: According to the calculations, the best effect (minimum IC5s) on the HT-29,A549 and MCF-7 cell line was related to the 3,4C compound. The ICso values were108, 77, and 69 pg I ml, respectively. The amount of ICso, for other two compoundswere below 200 pg / ml for all three cell line. The ICso values for 4C3M and 3C4Fcompounds on the HT29 cell line were 158 and 198 1pg I ml, A549,149 and l2l pg I ml,and MCF-7 was 187 and 110 trtg I ml, respectively.Conclusion: All three tested compounds showed cytotoxicity on three cell lines. Thebest compound was 3.4C, which contained IC56 less than 100 pg I ml for both the 4549and MCFT cell lines, and slightly higher than 100 for HT-29.Keywordsz invitro, cytotoxicity, imidazole, cell line, MTT

بررسی سمیت سلولی تعدادی از ترکیبات استخلاف شده در موقعیت متا و پارای فنیل ، فنوکسی اتیل ۵- نیترو -۲- متیل ایمیدازول بر روی رده های سلولی A۵۴۹ , MCF-۷ و  HT-۲۹

بررسی سمیت سلولی تعدادی از ترکیبات استخلاف شده در موقعیت متا و پارای فنیل ، فنوکسی اتیل ۵- نیترو -۲- متیل ایمیدازول بر روی رده های سلولی A۵۴۹ , MCF-۷ و HT-۲۹

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