Pouryazdanpanah, Noushin

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,- itt. t-),(yrg)b$p:[K,,// t)udtl(Po?t,, S,:",.t ,., o,r(*ill.lu rt v-?nJ- .s:q o u+lK4U3T ,r-t-u .r.ri dJl (J-l'..i.JlS ttu <-L;Ltg:,llJ:.jJi Jl ori 6lr:*l ,r-i,:.:|j," cS.r[1.1 cgl, JJ* ,S*f ,-rl.f o b .Jj1, e)r:;lrs:-lr;* 1l o.ri GIJA:*I d#il|}^ c5J+ cst, JC* ! *,lG- ;.r ,r-t+=-ot'.i'.lr;;3q .i*.;3.r : L. ..$.rlj: ,r-;G t;J+ tS.r :L^'.^l; .rl:.,.tJE!<JS+ .r*t* ;iS.r .,r.1-3.i';.r .,Jc' ,:S" :;rl .1, o ,i:l*l\f11-\YlV :.J*,--: Jt*\ . o :<.oL ;U+ ,SrKerman University of Medical SciencesFaculty of MedicineIn Partial Fulfillment of Requirements for the Degree (MSc)Title:The study of characteristics and differentiation ability of mesenchymal stemcells extracted from peripheral blood in comparison to mesenchymal stemcells extracted from bone marrowBy:Noushin PouryazdanpanahSupervisor:Dr. Alireza FarsinejadAdvisors:Dr. Ali DerakhshaniDr. Mahin NikougoftarYear: 2017a&it "ik; .51-o o:.; q,p.b ,r,-Ul4 af rj:"a jli"l;.> .jl.o J+,,r^*ilp cf:t*.1 cSLo Jy'-:eil.Sl 9&l C+.)9l.,i .r o.:Uh,l c^itr .*t*e 1r b ;ijl ,1" Jr- dr.l ,J..1 ,Sla .fks { 4+9', tt ..r;;l.r,r.l;b 1.1aS .c."1 eF )1f *i )sr i Afr.t 6t.;l9x;lF --U { 4+/ tr.c*,1 Jl1*."'F- l^ J/- Ul .s.J/- .siL;' .jli 9 t^ 6'is ,W "r"..s.! o*l fl+l ;l .ira .rf o;Lil J.D* dp q ,_tli ,* b Oi .r1l oxi el);bl -4ly A:Q 6b Jl- L. a*'.\i,, p u!^>* Of j o.t3 61g.,*,1 ,"^r*;lr. 6:l*...1o.uj ls of G\.,'.rn" ..r.,-r,5ls ,o$ $f ;r"*>, ;9>9 ;ly;a*'l;;. of jl.t J{.t, .(J U:3;le.r-.sl,rr.$ orlc'-"lReal Time PCR 9 6**-l*,* jl t J+ 6h3 N ,Sly.4 &,,,.,:4*r.ri, orbqe 9 o:lr ;Lj Ol9*:-l 9 G*.rtn J+ * ,yye 6i.U; to." ,: l1 to .1i ,t" Jr- 6kL; ,Jly.$ o)la;,rl G,-Ldtl.sG,-rFi &s )' :*2l o-u 1.,:.rt^ J/- qUu Jb*. Jf ) ol; l-s.rt J/- aJ rr.: ,r. JL;i oroi ,"..ur., p_L :sLs.i, CD45 b ;l s c..l" CD105 ,CD73 ,CD90 c*- d)li..t .rL J/- * byr 6Gf $ F )l ,l{:.o-, { ;.Lj d4,..tLi i s ot.f gL l, ,r^"sl;. .srt*r .rt^ J+ 4. Ly.r.rt Oj l^ J/- .1,1 o9),{c a,::-;\+Jl-.S;t-lq c"q o:lu-l )sf ,_its a5 "r";l orlr;Li a*llt" 9rl .1: ogVc a1 .rj;b ls C* 9;l9c=il .5Lr.r.i! .r Jb- Jf ).r."*t;l; cS:tri dlb Jy'- .S;Ll+ c.ea OJ-t. S &.p * oyp ,-f S; &_;,bL..llr 6 $l9r .r cj)\+_,, ,-fi1: g &.n * Al* e.5.- ijli e &,tu Of ZLSF 4+P 9 rJ."l";lt{ .,^.iiJli .fll*r cSta J/-.StLlq.s,,Llr+,;1; .;19*;1 ;;., )".>- .-igr,cr^i*iili. .rl[-t .j1." J+ :d J*15 O'.oiAbstract:Background and Objectives: The main rout of isolation of the stem cells is bone malrowaspiration, which is an invasive and painful rnethod. Recently, peripheral blood (PB) has beenused as an alternative source for MSCs isolation. Normally, a stimulating factor (mainlyGranulocyte-colony stimulating factor (GCSF)) is used to increase bone marrow stem cellmigration into the PB. Using GCSF is expensive, time consuming and cause side effects. Theaim of this study is to isolate MSCs from PB without using GCSF.Methods: Using ficole, mononuclear cells have been isolated from 10 milliliter sample of freshblood of a healthy and young female volunteer. These cells were cultured by cell concentrationmethod. Flow cytomtery and real-time PCR were used for immunophenotyping and evaluatinggene expression on the studied cells, respectively. To investigate the differentiation potential ofthese cells into fat cells and the bone, culturing mediums specific to differentiation were applied.Results: The PB derived MSCs positively expressed of CD90, CD105 and CD73, but, werenegative for CD45. Also, the level of expression of the genes Nanog, OcTL,Nucleostemin andB-Actin were high and similar to that of bone marow derived MSCs. The studied cells showedthe differentiation potential to differentiate into bone or fat cells.Conclusion: The cell concentration method can be used as a more convenient, affordable, andsafer method with few if any side effects, to prepare PB stem cells for therapeutic purposes.Keywords: Mesenchymal stem cells, Peripheral blood, Bone marrow, Differentiation, Isolation

The study of characteristics and differentiation ability of mesenchymal stem cells extracted from peripheral blood in comparison mesenchymal stem cells extracted from bone marrow

The study of characteristics and differentiation ability of mesenchymal stem cells extracted from peripheral blood in comparison mesenchymal stem cells extracted from bone marrow

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