Castro, Ana

Costa, Mariana

Meneses, Tânia

Paula Teixeira, Paula

Silva, Joana

English

Repositório Institucional da Universidade Católica Portuguesa

 1                   PORTUGUESE CONGRESS OF  MICROBIOLOGY AND BIOTECHNOLOGY Micro Biotec‘13 6th – 8th December | Aveiro Portugal Abstracts Book  236   Clinical Microbiology  P173 ANTIBIOTIC PROFILE OF STAPHYLOCOCCUS AUREUS ISOLATED FROM HEALTH CARE PERSONNEL WORKING IN HEALTH CENTERS  Ana Castro1; Mariana Costa2; Tânia Meneses2; Joana Silva1; Paula Teixeira1  1CBQF - Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Dr. António Bernardino Almeida, 4200-072 Porto, Portugal;  2Universidade Católica Portuguesa  Staphylococcus aureus is known as a pathogen responsible for skin infections and invasive diseases such as meningitismeningitides or pulmonary infection and with significant levels of carriage among in the community. Resistance to antibiotics is a problem and methicillin-resistant S. aureus (MRSA) have been increasingly isolated all over the world. The objective of this work was to evaluate nasal and hands carriage MRSA as well as hands carriage by among the health care staff in health care centers. From various health care centers near Porto, 322 swab samples from the nasal cavity and hands of health professionals, including volunteer doctors, nurses and auxiliaries, were collected. Swabs were spread onto Baird-Parker Agar and characteristic colonies were further isolated in Mannitol Salt Agar. Characteristic colonies were selected and confirmed as for S. aureus. The susceptibility to oxacillin, penicillin and ampicillin and to antibiotics of other classes rather than betalactams, namely ciprofloxacin, gentamicin, rifampin, vancomicin, tetracycline, erythromycin, nitrofurantoin and chloramphenicol was also investigated. The presence of methicillin resistance gene (mecA) was also evaluated by multiplex PCR (detecting 16S rRNA, nuc and mecA genes). Enterotoxin genes sea - sej and TSST were also evaluated by multiplex PCR. Fifty-four S. aureus were isolated or from nasal cavity or from hands or from both sites in the same individual representing 16.8% S. aureus recovered. Twenty-seven individuals carried S. aureus in nasal cavity (8.4%) while eight carried S. aureus both in nose and hands (2.5%). Eleven different individuals carried S. aureus only in the hands (3.4%). The prevalence of MRSA in 322 samples was only 6%, considering thatand 35.2% of the S. aureus isolates were MRSA. The most prevalent resistance among S. aureus was to beta-lactams and erythromycin. Resistance towhile to vancomicin and to gentamicin S. aureus did not show any resistancewas notfound. The most prevalent profile of enterotoxin genes was secbov seg sei.   

Antibiotic profile of staphylococcus aureus isolated from health care personnel working in health centers

https://repositorio.ucp.pt/bitstream/10400.14/14367/1/Antibiotic%20profile%20of%20staphylococcus%20aureus%20isolated%20from%20health%20care%20personnel%20working%20in%20health%20centers.PDF

Antibiotic profile of staphylococcus aureus isolated from health care personnel working in health centers

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Repositório Institucional da Universidade Católica Portuguesa