Costa, A

Costa, MJ

Lamas, S

Olsson, IAS

English

Repositório Aberto da Universidade do Porto

Global rating scales to assess the competence of researchers in handling and performing substance administrations in laboratory rodents Andreia Costa1,2,3, Sofia Lamas1,2, Manuel João Costa4, I. Anna S. Olsson1,2 1  i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal 2  IBMC - Institute for Molecular and Cell Biology, University of Porto, Portugal 3  ICBAS - Abel Salazar Institute of Biomedical Sciences, University of Porto, Portugal 4 ICVS - Life and Health Sciences Research Institute, School of Medicine, University of Minho, Portugal    This work is licensed under the Creative Commons Atribuição- NãoComercial 4.0 Internacional License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc/4.0/.  Index Instructions to the assessor                        2.  Approval criteria         3 Mouse restraint          4 Rat restraint          5 Mouse intraperitoneal administration         6 Rat subcutaneous administration       7 Station material         8       2 Instructions to the assessor    1. Ensure that the candidate’s identification (name and course/assessment reference) and the date of task performance are correct.  2. Provide clear indications to the candidate regarding the task required at each assessment (see the instructions to the candidate indicated in the different global rating scales). 3. Ensure that the candidate is wearing appropriate protective equipment before starting the demonstration. 4. Observe the candidate without interfering or communicating, except if the candidate expresses attitudes or behaviors that might compromise animal well-being, in which case the demonstration should be immediately halted, and the candidate’s performance should be considered “not approved”. 5. Use the scale to assess the candidate’s performance by selecting for each descriptor the categories representing the candidate’s actions. 6. Feedback may be given to candidate with focus on both the positive and negative aspects of the demonstration, to promote skills development.   7. Please note that information that is considered relevant, such as any special condition of the station that constrains the candidate’s performance, the animal strains used, disturbances occurring during the assessment, etc., should be registered under the “Observations” column.                  3 Suggested approval criteria for summative examinations  We recommend adjusting the approval criteria to the context of the assessment, considering institutional standards and policies, the objectives of the assessment, and the intended proficiency of the candidates. Such criteria should be defined prior to examinations and be transparent to candidates.   The pass criteria recommended for candidates who concluded formal training in laboratory animal science is a final score of [pass] for “global impression”, with at least one [clear pass] record for all the remaining items.    We propose that these pass criteria correspond to level 2/3 of supervision, as defined in the EU working document1. This score indicates that, although the candidate developed the skill, training with supervision is required to achieve mastery (level 0 of supervision, defined in the EU working document 1)        1  "The level of supervision can be divided in five stages: 4 – Supervisor present when the procedure takes place providing direct supervision and advice 3 – Supervisor aware when procedures are taking place and available for rapid intervention if required (i.e. in the vicinity of the procedure) 2 – Supervisor aware when procedures are taking place and available to attend to provide advice if required (i.e. in the vicinity of the establishment) 1 – Supervisor aware when procedures are taking place and available for discussion to provide advice if necessary (e.g. by telephone) 0 – No supervision required"   European Commission guidance document. National Competent Authorities for the implementation of Directive 2010/63/EU on the protection of animals used for scientific purposes. A working document on the development of a common education and training framework to fulfil the requirements under the Directive. Brussels, http://ec.europa.eu/environment/chemicals/lab_animals/pdf/guidance/education_training/en.pdf (2014, accessed 9 April 2021). page 14   4 Mouse restraint Candidate Name:                                                                                                             ID/ Course reference:                                  Instructions to the candidate:       1. Please remove one animal from the cage. 2. Perform the complete restraint.  3. Return the animal safely to the cage.            OBSERVATIONS:             Final score:   fail    pass   clear pass   Assessor signature:      Date:   Assessor Name: Assessment Station ID:    5 Rat restraint Candidate Name:                                                                 ID/ Course reference:                                  Instructions to the candidate:       1. Please remove one animal from the cage. 2. Perform the complete restraint. 3. Return the animal safely to the cage.            OBSERVATIONS:                Final score:   fail    pass   clear pass   Assessor signature:      Date:  Assessor Name: Assessment Station ID:    6 Mouse intraperitoneal administration Candidate Name:                                                                                                            ID/ Course reference:                                  Instructions to the candidate:       1. Please remove one animal from the cage,  2. Perform an intraperitoneal administration using the material in the station.  3. Return the animal safely to the cage.       OBSERVATIONS:              Final score:   fail    pass   clear pass   Assessor signature:      Date:  Assessor Name:Assessment Station ID:    7 Rat subcutaneous administration Candidate Name:                                                                                                              ID/ Course reference:                                  Instructions to the candidate:       1. Please remove one animal from the cage,  2. Perform a subcutaneous administration using the material in the station.  3. Return the animal safely to the cage.          OBSERVATIONS:             Final score:   fail    pass   clear pass   Assessor signature:      Date:    Assessor Name: Assessment Station ID:    8 Stations material Mouse restraint  cage with lid tunnel  Rat restraint  tunnel  Mouse intraperitoneal administration Sterile syringe (1 ml)  Sterile needle (25‐27 G; 5/8” length or less)  Saline for injection    Sharps container  Rat subcutaneous administration Sterile syringe (1 - 5 ml)  Sterile needle (23 - 25G; 5/8” length or less)  Saline for injected    Sharps container       

Global rating scales to assess the competence of researchers in handling and performing substance administrations in laboratory rodents

https://repositorio-aberto.up.pt/bitstream/10216/133419/1/GRS_SkillsAssessment_LAS_Rodents.pdf

Global rating scales to assess the competence of researchers in handling and performing substance administrations in laboratory rodents

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Repositório Aberto da Universidade do Porto