Antunes, Patrícia

Carvalho, A.P.

Costa, P.M.

Freitas-Silva, J.

Inácio, A.

Mourão, J.

Peixe, L.

English

Open Repository of the University of Porto

Occurrence of mcr-1 in Escherichia coli from rabbits of intensive farming

Repositório Aberto da Universidade do Porto

CONGRESS OF MICROBIOLOGY AND BIOTECHNOLOGY 2019December 5th-7th, 2019University of Coimbra (Pólo II)BOOK OF ABSTRACTS340 III6. Health Microbiology and Biotechnology P275. Occurrence of mcr-1 in Escherichia coli from rabbits of intensive farming Joana Freitas-Silva1, Ângela S. Inácio1, Joana Mourão2, Patrícia Antunes3, André Pinto de Carvalho4, Luísa Peixe2, Paulo Martins da Costa1 1 ICBAS, UP; CIIMAR, UP 2 UCIBIO/REQUIMTE, FFUP 3 UCIBIO/REQUIMTE, FFUP; FCNAUP 4 ICBAS, UP E-mail: joanafreitasdasilva@gmail.comBackground: The emergence of mobile colistin resistance genes (mcr) is yet another challenge in the fight against antimicrobial resistance, with reports proving the dissemination of these genes in different countries and different environments, including food producing animals. Rabbit intensive farming is highly dependent on the use of antibiotics, making this animal species relevant in the epidemiology of antimicrobial resistance. Objectives: Three colistin-resistant E. coli strains recovered from intestinal content of necropsied meat rabbits reared in two intensive production systems in Portugal were characterized. The isolates were recovered in 2015 and 2016 during routine diagnostic. Methods: Antibiotic susceptibility profiles were determined by disk-diffusion or reference broth microdilution method (colistin). The isolates were screened for mcr-(1-5) genes by PCR and sequencing. Plasmid characterization (PCR-PBRT/pMLST/sequencing) and location (S1-PFGE- hybridization) were performed. Isolates were subjected to conjugation assays using the recipient strain E. coli HB101. Clonal relatedness was assessed by MLST. Results: These isolates were retrieved from three samples received in our laboratory in 2015 and 2016, amongst a total of 13 samples collected from five commercial farms. Two isolates were recovered from samples of kits fed with a commercial diet medicated with colistin, yet, one isolate was from a rabbit not exposed to colistin. All isolates were multidrug-resistant and presented colistin minimal inhibitory concentrations ranging from 4 to 8 µg/mL. They belonged to different clonal lineages and mcr-1 was carried in IncHI2/ST2 (clones ST1589 in farm I and ST206 in farm II) or IncHI2/ST4 (clone ST1431 in farm II) plasmids, non-transferable by conjugation assays. Acknowledgements: This work was developed under the Strategic Funding UID/Multi/04423/2019 and Project No. POCI-01-0145-FEDER-028736, co-financed by COMPETE 2020, Portugal 2020 and the European Union through the ERDF, and by FCT through national funds. 

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Occurrence of mcr-1 in Escherichia coli from rabbits of intensive farming

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