Antunes, Patrícia

Duarte, B.

Ferreira, C.

Freitas, A.R.

Mourão, J.

Novais, C.

Peixe, L.

Rebelo, A.

Ribeiro, S.

Scholes, C.

English

Open Repository of the University of Porto

High rates of ampicillin-multidrug-resistant Enterococcus faecium in chicken meat from Portugal

Repositório Aberto da Universidade do Porto

P2547 High rates of ampicillin multidrug-resistant Enterococcus faecium in chickenmeat from PortugalCarla Novais*1, Ana Raquel Freitas1, Carolina Ferreira2, Christopher Scholes2,3, Barbara Duarte1, Sofia Ribeiro2,Joana Vanessa Cordeiro Melro Mourão1, Andreia Rebelo1,4, Patricia Antunes1,5, Luisa Maria Vieira Peixe11 UCIBIO/REQUIMTE. Faculty of Pharmacy. Biological Sciences Department., University of Porto, Porto, Portugal, 2Faculty of Pharmacy. Biological Sciences Department., University of Porto, Porto, Portugal, 3 University ofStrathclyde, Glasgow, United Kingdom, 4 Escola Superior de Saúde. , Instituto Politécnico do Porto. , Porto,Portugal, 5 Faculty of Food Sciences and Nutrition, University of Porto, Porto, PortugalBackground: Resistance to ampicillin-AmpR is associated with Enterococcus faecium-Efm from hospitalized-humans (clade-A1) and at lesser extent with community-based isolates (clade-A2: human and animal strains;clade-B: human-commensal strains). Recently, AmpR combined with specific putative virulence factors wereproposed as molecular markers of Efm linked to human infections, which can have an impact in Efm riskassessment in different public health contexts (PMID:29519512). Here we evaluated the occurrence of AmpR-Efmwith potential public health impact in chicken-meat samples.Materials/methods: Pooled chicken-meat samples (n=53; neck skin of 10 carcasses of the same batch each; 29producers) obtained after slaughter and chilling at processing level were collected during 6 months of 2018.Samples (25g) were pre-enriched in Buffered-Peptone-Water (37ºC/16-18h), enriched in BHI-broth+Amp-16µg/mL (37ºC/24h) and plated in Slanetz-Bartley-(SB) and SB+Amp-16mg/L (37ºC/48h). Susceptibility wasstudied for 11 antibiotics (disk-diffusion; EUCAST/CLSI) in one Efm per sample/plate. Species identification (ddl),search of virulence factors (surface-proteins:esp/sgrA/ecbA/complete-acm; enhancedcolonization:hyl/ptsD/orf1481; genomic plasticity:IS16) and copper-tolerance (tcrB/cueO) genes were searched byPCR. Linezolid resistant genes (optrA, poxtA) were directly searched in DNA extracted from the pre-enrichment byPCR. Clonality was evaluated in representative isolates by SmaI-PFGE and MLST.Results: AmpR-Efm were present in 83% (n=44/53) of samples. Isolates (n=49) were mostly recovered fromSB+Amp plates (n=45/49-92%). AmpR-Efm were co-resistant to tetracycline, erythromycin (both 100%-n=49/49),quinupristin-dalfopristin (94%-n=46/49), ciprofloxacin (73%-n=36/49), streptomycin (31%-n=15/49), gentamicin(12%-n=6/49) or chloramphenicol (2%-n=1/49), all multidrug-resistant (MDR). Glycopeptides or linezolid(including the presence of optrA/poxtA in samples) resistance was not observed. By PFGE, 10 clones wereidentified, some persisting for long periods and from diverse producers (PFGE-A:7-isolates/7-samples/6-producers/5-months; C:10-isolates/10-samples/10-producers/5-months; B:2-isolates/2-samples/2-producers/2-months; G:3-isolates/3-samples/3-producers/2-months). The most widespread ones, A and C, were identified asST462 and ST1091, respectively (both clade-A2). The ptsD was detected in clone G (n=1) and acm in clones C, G, L,I and J (n=5). The tcrB/cueO were not detected.Conclusions: A high rate of AmpR-MDR-Efm was recovered from recent chicken-meat-samples, suggesting ahigh selective pressure by beta-lactams in the poultry production setting. The evaluation of the pathogenicpotential of the AmpR-Efm widespread clones is required, as they do not carry virulence genes combinations(PMID:29519512) often associated with human infection’s strains.29TH ECCMID13-16 APRIL 2019 AMSTERDAM, NETHERLANDS POWERED BY M-ANAGE.COM 

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High rates of ampicillin-multidrug-resistant Enterococcus faecium in chicken meat from Portugal

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