Transthyretin participates in beta-amyloid transport from the brain to the liver- involvement of the low-density lipoprotein receptor-related protein 1?
Transthyretin (TTR) binds Aβ peptide, preventing its deposition and toxicity. TTR is decreased in
Alzheimer’s disease (AD) patients. Additionally, AD transgenic mice with only one copy of the TTR
gene show increased brain and plasma Aβ levels when compared to AD mice with both copies of the
gene, suggesting TTR involvement in brain Aβ efflux and/or peripheral clearance. Here we showed that
TTR promotes Aβ internalization and efflux in a human cerebral microvascular endothelial cell line,
hCMEC/D3. TTR also stimulated brain-to-blood but not blood-to-brain Aβ permeability in hCMEC/D3,
suggesting that TTR interacts directly with Aβ at the blood-brain-barrier. We also observed that TTR
crosses the monolayer of cells only in the brain-to-blood direction, as confirmed by in vivo studies,
suggesting that TTR can transport Aβ from, but not into the brain. Furthermore, TTR increased Aβ
internalization by SAHep cells and by primary hepatocytes from TTR+/+ mice when compared to
TTR−/− animals. We propose that TTR-mediated Aβ clearance is through LRP1, as lower receptor
expression was found in brains and livers of TTR−/− mice and in cells incubated without TTR. Our
results suggest that TTR acts as a carrier of Aβ at the blood-brain-barrier and liver, using LRP1