Laser-assisted fluorescence detection of plaque.

Abstract

Selective fluorescence-marking of plaque offers new possibilities in cardiovascular diagnosis and therapy. Angioscopic investigations and spectrometry-assisted laser angioplasty will be simplified and more effective as compared with methods of today. It might help to make laser angioplasty a further promising interventional method to overcome, at least partially, the problems caused by atheromatous or atherosclerotic changes in the cardiovascular system. Fluorescence detection and imaging of markers is usually limited by the intrinsic fluorescence of tissue. Optical differential methods in combination with two-wavelength laser excitation and computer-assisted image processing, however, allow for discrimination of background-related signals and enable plaque detection and imaging at a high contrast. Plaque consists of either fibrotic, lipoid, or calcified depositions and is rather bradytrophic. For that reason in vitro experiments on human specimens post mortem seem to be justified and of clinical evidence. Due to intrinsically different fluorometric properties of plaque and normal vascular tissue imaging of marker-free plaque areas is possible. Additionally the specimens have been incubated with a haematoporphyrin-containing fluorescence marker at concentrations of 10-40 μg ml -1 and incubation times of 60 min in order to obtain a corresponding increase in contrast. Lipoid depositions show the highest contrast because of lipophilic properties of the marker, while fibrotic and calcified plaque is slightly less effectively marked. The results, however, so far obtained indicate that fluorescence detection of plaque promises further progress in diagnosis and therapy of cardiovascular diseases