The occurrence ofAnaplasma phagocytophilumwas investigated in spleen and serum samplesfrom Swedish moose (Alces alces) in southern Sweden (island and mainland). Samples wereanalysed for presence ofA. phagocytophilumDNA by real-time PCR (n=263), and forAnaplasmaantibodies with ELISA serology (n=234). All serum samples had antibodies againstA. phagocytophilum. The mean DNA-based prevalence was 26·3%, and significant (

Aspan, A.

Dalin, A.-M.

Gavier Widén, D.

Hutchings, M. R.

Malmsten, J.

Rydevik, G.

Stuen, S.

Söderquist, L.

Thulin, C.-G.

Yon, L.

Epidemiology and Infection

PubMed

SUMMARYThe occurrence ofAnaplasma phagocytophilumwas investigated in spleen and serum samples from Swedish moose (Alces alces) in southern Sweden (island and mainland). Samples were analysed for presence ofA. phagocytophilumDNA by real-time PCR (n = 263), and forAnaplasmaantibodies with ELISA serology (n = 234). All serum samples had antibodies againstA. phagocytophilum. The mean DNA-based prevalence was 26·3%, and significant (P &lt; 0·01) temporal, and spatial variation was found. Island moose had significantly (P &lt; 0·001) higher prevalence ofA. phagocytophilumDNA than moose from the mainland areas. Two samples were sequenced to determine genetic variation in the 16S rRNA andgroESLgenes. Genetic sequence similarity with the human granulocytic anaplasmosis agent, equine granulocytic ehrlichiosis agent, and different wildlife-associatedA. phagocytophilumvariants were observed in the 16S rRNA andgroESLgenes. Our study shows that moose are exposed toA. phagocytophilumin Sweden, and represent a potential wildlife reservoir of the pathogen.</jats:p

J. MALMSTEN

D. GAVIER WIDÉN

G. RYDEVIK

L. YON

M. R. HUTCHINGS

C.-G. THULIN

L. SÖDERQUIST

A. ASPAN

S. STUEN

A.-M. DALIN

Crossref

English

Temporal and spatial variation inAnaplasma phagocytophiluminfection in Swedish moose (Alces alces)

The occurrence of Anaplasma phagocytophilum was investigated in spleen and serum samples from Swedish moose (Alces alces) in southern Sweden (island and mainland). Samples were analysed for presence of A. phagocytophilum DNA by real-time PCR (n=263), and for Anaplasma antibodies with ELISA serology (n=234). All serum samples had antibodies against A. phagocytophilum. The mean DNA-based prevalence was 26·3%, and significant (P&lt;0·01) temporal, and spatial variation was found. Island moose had significantly (P&lt;0·001) higher prevalence of A. phagocytophilum DNA than moose from the mainland areas. Two samples were sequenced to determine genetic variation in the 16S rRNA and groESL genes. Genetic sequence similarity with the human granulocytic anaplasmosis agent, equine granulocytic ehrlichiosis agent, and different wildlife-associated A. phagocytophilum variants were observed in the 16S rRNA and groESL genes. Our study shows that moose are exposed to A. phagocytophilum in Sweden, and represent a potential wildlife reservoir of the pathogen

Malmsten, J

Gavier Widen, D

Rydevik, G

Yon, L

Hutchings, MR

Thulin, CG

Soderquist, L

Aspan, A

Stuen, S

Dalin, AM

SRUC - Scotland's Rural College

Temporal and spatial variation in Anaplasma phagocytophilum infection in Swedish moose (Alces alces)

Gavier Wid�n, D.

S�derquist, L.

Repository@Nottingham

Temporal and spatial variation in Anaplasma phagocytophilum infection in Swedish moose (Alces alces)

https://www.cambridge.org/core/services/aop-cambridge-core/content/view/B55B375F1919FA6358BC5ABC8FF25B16/S0950268813002094a.pdf/div-class-title-temporal-and-spatial-variation-in-span-class-italic-anaplasma-phagocytophilum-span-infection-in-swedish-moose-span-class-italic-alces-alces-span-div.pdf

Temporal and spatial variation in Anaplasma phagocytophilum infection in Swedish moose (Alces alces)

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SRUC - Scotland's Rural College

Repository@Nottingham

SRUC - Scotland's Rural College