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6-BA、NAA对苜蓿不同外植体愈伤组织培养的影响
Authors
张博
王彩虹
+4 more
王玉祥
肖燕
范永刚
赵红娟
Publication date
1 January 2007
Publisher
Abstract
以新牧1号和新疆大叶两个苜蓿品种为试材,研究了7个培养基对不同无菌苗外植体愈伤组织诱导和生长影响,以及不同浓度的植物生长调节物质6-苄氨基腺嘌呤(6-BA)和萘乙酸(NAA)对愈伤组织生长及分化的作用。结果表明:(1)浓度在2~8 mg/L,NAA有利于苜蓿愈伤组织的诱导,随浓度的增大呈促进作用,而在1~3 mg/L,1mg/L 6-BA有利于苜蓿愈伤组织的诱导,随浓度的增大呈抑制作用;(2)MS+2,4-D 2mg/L+NAA 4 mg/L+6-BA 1 mg/L即MS4为新牧1号的较佳诱导培养基,MS+2,4-D 2 mg/L+NAA 8 mg/L+6-BA 1 mg/L即MS5为新疆大叶苜蓿的较佳诱导培养基;(3)以无菌苗下胚轴、子叶及叶片为外植体,确定下胚轴为诱导愈伤组织的最佳外植体
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Last time updated on 29/11/2016