Determination of calpastatin (CAST) gene polimorphism in some native sheep breeds reared in Turkey by PCR-RFLP method [Türkiye’de yetiştirilen bazı yerli koyun irklarında kalpastatin (CAST) geni polimorfizminin PCR-RFLP yöntemiyle belirlenmesi]

Abstract

In this study, calpastatin (CAST) gene polimorphism was investigated in 7 native sheep breeds reared in Turkey by using PCR-RFLP method. The frequencies of M and N alleles of CAST gene in Kangal (n= 31), Awassi (n= 26), Güney Karaman (n= 23), Akkaraman (n= 21), Morkaraman (n= 34), Karayaka (n= 33), and Karakas (n= 22) sheep breeds were determined as 0.92-0.08, 0.59-0.41, 0.67-0.33, 0.69-0.31, 0.87-0.13, 0.86-0.14, 0.89-0.11 respectively. According to chi-square test, all the other populations were consistent with Hardy-Weinberg equilibrium, whereas Morkaraman, İvesi and Karayaka populations showed significant (P<0.05) deviation from Hardy-Weinberg equilibrium for the CAST gene. © Ankara Üniversitesi Ziraat Fakültesi