Cai, J

HUA, Y

Huang, J

Kao, RYT

Lau, CY

Sze, KH

Yu, B

Yuen, KY

ZHANG, B

BMC Infectious Diseases

PubMed

Bao-Zhong Zhang

Jianpiao Cai

Bin Yu

Yanhong Hua

Candy Choiyi Lau

Richard Yi-Tsun Tsun Kao

Kong-Hung Sze

Kwok-Yung Yuen

Jian-Dong Huang

Crossref

A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile

HKU Scholars Hub

Abstract Background Clostridium difficile-associated disease (CDAD) constitutes a great majority of hospital diarrhea cases in industrialized countries and is induced by two types of large toxin molecules: toxin A (TcdA) and toxin B (TcdB). Development of immunotherapeutic approaches, either active or passive, has seen a resurgence in recent years. Studies have described vaccine plasmids that express either TcdA and/or TcdB receptor binding domain (RBD). However, the effectiveness of one vector encoding both toxin RBDs against CDAD has not been evaluated. Methods In the study, we constructed highly optimized plasmids to express the receptor binding domains of both TcdA and TcdB from a single vector. The DNA vaccine was evaluated in two animal models for its immunogenicity and protective effects. Results The DNA vaccine induced high levels of serum antibodies to toxin A and/or B and demonstrated neutralizing activity in both in vitro and in vivo systems. In a C. difficile hamster infection model, immunization with the DNA vaccine reduced infection severity and conferred significant protection against a lethal C. difficile strain. Conclusions This study has demonstrated a single plasmid encoding the RBD domains of C. difficile TcdA and TcdB as a DNA vaccine that could provide protection from C. difficile disease

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http://hub.hku.hk/bitstream/10722/248315/1/content.pdf

A DNA vaccine targeting TcdA and TcdB induces protective immunity against Clostridium difficile

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HKU Scholars Hub

Directory of Open Access Journals