Two of the genes controlling nodulation response in soybeans were tested for linkage associations with genes controlling pubescence color (T) and flower color (W1 ), chlorophyll deficiency (y9 ) and absence of pubescence (P). The rj1 gene (W1lliams and Lynch, 1954) in homozygous recessive condition results in a non-nodulating phenotype with a broad spectrum of Rhizobium japonicum strains. The dominant gene, Rj4 (Vest and Caldwell, 1972) conditions an ineffective nodulation response when inoculated specifically with R. japonicum strain 61 of the Beltsville Culture Collection