Research Notes : Studies in polyploidy in soybeans: A simple and effective colchicine technique of chromosome doubling for soybean (Glycine max (L .) Merr.) and its wild relatives.

Abstract

Tang and Loo (1940) first reported the induction of tetraploid soybeans by soaking day-old seedlings in 0.05 to 0.1% colchicine solution for 24 or 48 h. Oinuma (1952) obtained tetraploids by soaking dry soybean seeds in 0.1% colchicine solution for 24, 48 and 72 h. His results showed that the survival of resulting plants was poor