Lembaga Penelitian dan Pengabdian Kepada Masyarakat, Universitas Surabaya
Abstract
The plants characteristics after transforming intended to show that the success of transforming,
therefore, screening methods of transformed population is needed. Selection methods had been
done 5 cycles for 3 weeks at the medium of hygromycin. This methods was preliminary methods
which could be used to identify the transformed plants which carried SoSUTI gene, which was
transformed in the plants because that gene was bound together with hpt gene which expressed
phosphotransferase enzyme for endurance towards hygromycin antibiotic. The hygromycin
concentration used to do transformed screening was determined by growing non-transformant
sugarcane shoot at the MS medium with several hygromycin concentrations (0-40 mg/l). Hygromycin
concentration, which was capable to kill the sugarcane shoot, was used further for selecting the result
of Agrobacterium tumefacieris GV 3010 mediated transformed genes in the sugarcane shoot. The 15
mg/1 hygromycin concentration was used to do transformed screening for 2 cycles of selection, and it
would be done further on 3, 4 and 5 cycles of selection using hygromycin 20, 25 and 30 mg/l. From
the result of 500 explants shoot transformation, 41 shoots was able to grow at the selection medium
with the transformation effective of 8.2 %
