Karakteristik Fisiologis Ralstonia Solanacearum Penyebab Penyakit Layu Bakteri Nilam

Abstract

Physiological characteristics of Ralstonia solanacearumcausing bacterial wilt disease on patchouli plantThe study of characteristics of Ralstonia solanacearum causingbacterial wilt disease on patchouli plant was conducted in the patchouliplant field in Pasaman Barat West Sumatera and bacteriological laboratoryand green house of Agricultural Faculty of Gadjah Mada University,Yogyakarta. The field activity were identification of disease symptom andcollection of infected plant by bacterial disease from the patchouli plantfield that have the height disease intensity (more than 75%) that wasconducted on January 2003. Activity of laboratory and green house wereisolation and assay of bacterial morphology, hypersensitive andpathogenicity test, bacteriological characteristic, fluorescens pigment,antibiotic, biotype and ras pathology were conducted from January toAugust 2003. Results showed that 31 isolates showed hypersensitivereaction on tobacco leaf. Twenty isolates infected patchouly plant withwilt symptoms with incubation period 14.6 – 39.3 days after inoculation.Ns 31 was the most virulent isolate. Analytic results of bacteriologicalcharacteristic showed that the bacterial isolates of Patchouli plant fromWest Pasaman-West Sumatera is Ralstonia solanacearum. Based onbiotype and host range test, this isolates was grouped into biotype III andras one