Pengaruh Media Dan Zat Pengatur Tumbuh Terhadap Multiplikasi Tunas Selasih (Ocimum Basilicum) in Vitro

Abstract

Effect of media and growth regulator on shootmultiplication of ocimum (Ocimum basilicum) in vitroOcimum (Ocimum basilicum) is one of important essential oil plantsin Indonesia which is generally used as medicine or pesticide. For plantdevelopment and genetic variants improvement, tissue culture propagationwas conducted. The studies was conducted in March to December 2004 atthe Tissue Culture Laboratory of the ISMECRI Bogor. Plant materialsused were ocimum explants apical shoot taken from a green house.Treatments tested were the effect of physical media and concentrationlevel of Benzyl Adenin (BA). The experiment used a completelyrandomized design with two factors. First factor was physical media (solidand liquid) and the second factor was BA concentrations (0.1 ; 0.3 and 0.5mg/l ). Each treatment consisted of ten replications. The parametersobserved were number of shoots, shoot length, number of leaves and rootsand culture performance, nine weeks after culturing. Research resultshowed that the use of solid media in combination with 0.3 mg/l BA wasthe best media for shoot multiplication of ocimum in vitro and it was notsignificantly different with liquid medium enriched with BA (0.1 -0.5mg/l). Shoot length was only affected by BA concentration and the longestshoot was obtained by BA 0.3 mg/l but it was not significantly differentwith BA 0.1 mg/l. Both treatments had no effect on the number of leavesand roots