Sato, Kevin Y

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https://ntrs.nasa.gov/search.jsp?R=20120014406 2019-08-30T22:12:03+00:00Z
Cell Bio Tech Demo Team 
• NASA ARC ISS Utilization Office - Functionality Projects 
- NASA MSFC (Ops and Crew Training) 
- JSC (PIM) 
• Tissue Genesis, Inc. (CBTD Hardware Developer) 
Bioculture System for ISS 
• NASAARC ISS Utilization Office is developing the next generation incubator that 
will be dedicated to life science space flight research: 
- Mammalian and non-mammalian cell cultures (adherent, suspension, 3D) 
- Microbiology cultures (prokaryotic and eukaryotic) 
• Capabilities 
- Multiple independent experiment sub-housings 
- Closed loop flow path system allowing medium oxygenation and profusion 
- Independent temperature control 
- 3 levels of containment (closed loop plus 2 additional covers) 
- Automated systems for injection of fixatives, factors, etc. 
- Automated environmental gas supply (not independent) 
- Crew access for on-orbit experiment initiation, change-out of specimens and 
media bags, specimen injection into biospecimen chambers, sub-culturing, 
sampling, factor injection, and use of other ISS science facilities 
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Cell Bio Tech Demo Objectives 
1. Demonstrate that the tools and procedures used to introduce or remove 
specimens from a medium bag can be completed on-orbit without the 
introduction of a microbial contaminant into the liquid medium 
2. Demonstrate the on-orbit functional compatibility of Bioculture Systems 
components and tools with ISS Wetlab kit items 
3. Demonstrate the introduction of a biological specimen into the medium bag 
and sealing of flow paths using candidate tools 
4. Demonstrate use of a candidate tubing cutter and crimp tubing for sealing and 
maintaining sterility on-orbit 
5. Obtain crew feedback concerning BIOS components and their on-orbit 
operation, utilize that feedback in the development of BIOS crew procedures. 
6. Support NASA education/outreach by distributing the tech demo biological 
samples obtained as a result on-orbit operations 
Cell Bio Tech Demo Launch and Operations 
SpX-2 Launch: 12/12 
Return: 1/13 
On-Orbit Operations 
1) Demonstrate use of transfer tool and specimen container and maintenance of 
sterility; demonstrate new crimp cut tubing and cutter 
2) Demonstrate transfer of medium from one bag to another bag and maintenance of 
sterility; demonstrate new crimp cut tubing and cutter 
3) Demonstrate transfer of a "specimen" to an OptiCell for interface with an ISS 
processing facility 
4) Demonstrate transfer of a biological specimen (S. cerevisiae) to a media bag 
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Ops: Demonstrate use of transfer tool and specimen container and maintenance 
of sterility; demonstrate new crimp cut tubing and cutter 
Media Bag 
Nutrient Sample Tool 
~yringe + Stopcock + 
er) 
~~ ~ i / 
Media Transfer 
Container 
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Ops: Demonstrate transfer of medium from one bag to another bag and maintenance 
of sterility; demonstrate new crimp cut tubing and cutter 
or 
Patient 
Safe 
Syringe Media Bag 1 
J...~J... ~;pplge genes,s 
incorporated 
Media Bag 2 
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Ops: Demonstrate transfer of a "specimen" to an OptiCell for interface with an ISS 
processing facility 
OptiCell Sample Tool 
(Sy[inge + Dual Che«,.k Valve + Stopcock + Blunt 
die) 
OPtiCel/ 
Interface 
fray 
OptiCell 
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Ops: Demonstrate transfer of a biological specimen (S. cerevisiae) to a media bag 
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./ 
YPD Bag 
Yeast Sample Tool 
(Syringe + Stopcock + 
Holder) 
Crimp Tubing 
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N~~ 
Yeast Transfer 
Container 
Cell Bio Tech Demo POC: 
Nicole Rayl (Project Manager) 
Nicole.a.rayl@nasa.gov 
Kevin Sato (Project Scientist) 
Kevin .y.sato@nasa.gov 


Cell Bio Tech Demo

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