Here, we presented new original data on the effects of iron

depletion on the circulating lipid pro

fi

le in B6HCV mice, a murine

model of HCV-related dyslipidemia. Male adult B6HCV mice were

subjected to non-invasive iron depletion by low iron diet. Serum

iron concentration was assessed for evaluating the effects of the

dietary iron depletion. Concentrations of circulating triglycerides,

total cholesterol, Low Density Lipoproteins (LDLs), High Density

Lipoproteins (HDLs) were analyzed and reported by using stacked

line charts. The present data indicated that low serum iron con-

centration is associated to i) lower serum triglycerides con-

centrations and ii) increased circulating LDLs. The presented ori-

ginal data have not been published elsewhere

Amato, Rosario

Conigliaro, Alice

Costa, Viviana

Mancone, Carmine

Data in Brief

English

PubMed

Here, we presented new original data on the effects of iron depletion on the circulating lipid profile in B6HCV mice, a murine model of HCV-related dyslipidemia. Male adult B6HCV mice were subjected to non-invasive iron depletion by low iron diet. Serum iron concentration was assessed for evaluating the effects of the dietary iron depletion. Concentrations of circulating triglycerides, total cholesterol, Low Density Lipoproteins (LDLs), High Density Lipoproteins (HDLs) were analyzed and reported by using stacked line charts. The present data indicated that low serum iron concentration is associated to i) lower serum triglycerides concentrations and ii) increased circulating LDLs. The presented original data have not been published elsewhere

Alice Conigliaro

Viviana Costa

Rosario Amato

Carmine Mancone

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Data on the effects of low iron diet on serum lipid profile in HCV transgenic mouse model

Crossref

Here, we presented new original data on the effects of iron
depletion on the circulating lipid pro
fi
le in B6HCV mice, a murine
model of HCV-related dyslipidemia. Male adult B6HCV mice were
subjected to non-invasive iron depletion by low iron diet. Serum
iron concentration was assessed for evaluating the effects of the
dietary iron depletion. Concentrations of circulating triglycerides,
total cholesterol, Low Density Lipoproteins (LDLs), High Density
Lipoproteins (HDLs) were analyzed and reported by using stacked
line charts. The present data indicated that low serum iron con-
centration is associated to i) lower serum triglycerides con-
centrations and ii) increased circulating LDLs. The presented ori-
ginal data have not been published elsewhere

CONIGLIARO, Alice

MANCONE, Carmine

Archivio della ricerca- Università di Roma La Sapienza

Contents lists available at ScienceDirectData in BriefData in Brief 12 (2017) 22–25SMhttp://d2352-34(http://cn CorrE-mjournal homepage: www.elsevier.com/locate/dibData ArticleData on the effects of low iron diet on serum lipidprofile in HCV transgenic mouse modelAlice Conigliaro a,n, Viviana Costa b, Rosario Amato c,Carmine Mancone aa Dipartimento di Biotecnologie Cellulari ed Ematologia, Sapienza University of Rome, Rome 00185, Italyb Rizzoli Orthopedic Institute, Palermo, Italy, Innovative Technological Platforms for Tissue Engineering,Theranostic and Oncology, Palermo, Italyc Department of “Scienze della Salute”, University”Magna Græcia” of Catanzaro, Viale Europa, Catanzaro, Italya r t i c l e i n f oArticle history:Received 30 January 2017Received in revised form10 February 2017Accepted 6 March 2017Available online 18 March 2017Keywords:Iron depletionTriglyceridesLow density lipoproteinsx.doi.org/10.1016/j.dib.2017.03.02009/& 2017 The Authors. Published by Elsevreativecommons.org/licenses/by/4.0/).esponding author.ail address: conigliaro@bce.uniroma1.it (A.a b s t r a c tHere, we presented new original data on the effects of irondepletion on the circulating lipid profile in B6HCV mice, a murinemodel of HCV-related dyslipidemia. Male adult B6HCV mice weresubjected to non-invasive iron depletion by low iron diet. Serumiron concentration was assessed for evaluating the effects of thedietary iron depletion. Concentrations of circulating triglycerides,total cholesterol, Low Density Lipoproteins (LDLs), High DensityLipoproteins (HDLs) were analyzed and reported by using stackedline charts. The present data indicated that low serum iron con-centration is associated to i) lower serum triglycerides con-centrations and ii) increased circulating LDLs. The presented ori-ginal data have not been published elsewhere.& 2017 The Authors. Published by Elsevier Inc. This is an openaccess article under the CC BY license(http://creativecommons.org/licenses/by/4.0/).Specifications Tableubject area Biologyore specific sub-ject areaBiochemistry, Genetics and Molecular Biologyier Inc. This is an open access article under the CC BY licenseConigliaro).THDEEDFig.diffA. Conigliaro et al. / Data in Brief 12 (2017) 22–25 23ype of data1. Serum iron concentraerence. For details see MTable, figures, Text file.ow data wasacquiredAnalyses were performed on an automated haematology analyser using mouse-specific algorithms and parameters (ProCyte DX IDEXX and Abaxis VetScan5).ata format Raw, AnalyzedxperimentalfactorsB6HCV transgenic mice were from Markus Heim laboratory at Department ofBiomedicine University Hospital of BaselxperimentalfeaturesIron and lipids concentrations were measured in serum samples of B6HCV micefed low iron diet for thirty days.ata sourcelocationPalermo, Italyata accessibility Data are available with this articleDValue of the data The presented data indicated that low-iron diet i) reduced serum triglycerides concentrations andii) increased levels of circulating LDLs in HCV transgenic mouse model. No differences have beenfound on cholesterol and HDLs concentrations in low iron feed mice respect to controls. These data are the further evidence on the interplay between the iron and lipid metabolism. The data in this article could be useful to optimize further clinical trials to evaluate the potentialtherapeutic effect of dietary iron depletion on dyslipidaemia in patients with Hepatitis C infectionand hepatic iron overload.1. DataMice were fed a low-iron diet for thirty days and reduction in blood iron concentration wasassessed by biochemical analysis (Fig. 1). During the treatment B6HCV mice did not show any sign ofdistress. Compared with control group, low iron diet resulted in a significant reduction in circulatingtriglycerides (TGs), while there was no change in total cholesterol concentration (Fig. 2). Moreover,mice fed the low-iron diet showed increased levels of circulating LDLs respect to control group; nodifferences were observed in the concentrations of HDLs (Fig. 2). All the obtained results are sum-marized in Table 1.2. Experimental designTo date, many evidences showed that iron overload plays a causal role in the hepatic fat accu-mulation. In particular, studies on HCV (hepatitis-C-virus) transgenic mice fed an excess-iron diettion in B6HCV mice fed the normal (n.10) and low (n.10) iron diet. p value is indicated; * significantethods section.Fig. 2. Quantitative analysis of the indicated classes of lipids in the serum samples of B6HCV mice fed normal (n.10) and low(n.10) iron diet. p values are indicated; * significant differences. For details see Methods section.Table 1Biochemical results of B6HCV mice fed the normal (Ctr) and low iron diet (Lid).Iron mg/dl Triglycerides mg/dl Total cholesterol mg/dl LDL U/l HDL U/lCtr Lid Ctr Lid Ctr Lid Ctr Lid Ctr LidMean 117,8 77,60 31,67 19,17 78,29 75,00 5,057 8,480 65,29 63,33Minimum 100,0 45,00 12,00 16,00 45,00 51,00 0,8000 6,000 40,00 43,00Maximum 131,0 110,0 46,00 24,00 99,00 95,00 8,800 11,20 76,00 79,00Std. Deviation 10,42 26,63 13,38 3,371 17,79 15,80 3,093 2,373 12,84 13,71A. Conigliaro et al. / Data in Brief 12 (2017) 22–2524demonstrated that hepatic iron overload exacerbates the imbalance of lipid metabolism by bothlipogenesis activation and reduced oxidation activity of fatty acids [1,2]. Moreover, HCV-induced ironaccumulation contributes to liver steatosis by the inhibition of β-lipoproteins mediated fat export,thus leading hypobetalipoproteinemia [3,4]. In this study, male B6HCV-transgenic mice [5] expressingthe HCV polyprotein were fed a low-iron diet or a control diet for thirty days. Mice in each group wereassessed for blood iron concentration and circulating lipid profiling (triglycerides, total cholesterol,Low Density Lipoproteins (LDLs), High Density Lipoproteins (HDLs)).3. Materials and methodsB6HCV transgenic mice were from Markus Heim laboratory at Department of Biomedicine Uni-versity Hospital of Basel [5]. Mice were bred and maintained in accordance with the institutionalA. Conigliaro et al. / Data in Brief 12 (2017) 22–25 25guidelines of the University of Palermo Animal Care Committee. The experimental procedures havebeen communicated to the Ministry of Health in accordance with the ministerial directive at the timeof the experiments: legislative decree 116/92, annex 4 to the circular of the Ministry of Health n. 8 of1994. No suffering was inflicted on the animals before euthanasia. Twenty male B6HCV (6 monthsold) equally divided into 2 groups, were fed a low-iron diet (ssniff EF R/M iron deficient diet) orrelative control diet. After one month, mice were killed by CO2 asphyxiation and blood collected bypost-mortem cardiac puncture. No animals became severely ill or died prior to the experimentalendpoint. Euthanasia in mice was performed by carbon dioxide asphyxiation.Blood biochemical analysis were performed on an automated hematology analyzer using mouse-specific algorithms and parameters (ProCyte DX IDEXX and Abaxis VetScan5). Total cholesterol wasmeasured by the CHOD-PAP method adapted to automated analyzer (Chem 400), non-complexed HDLwas measured using the CHOD-PAP method. For triglyceride determinations GPO-PAP method wasused. Quantitative values are expressed as mean7SD (Table 1). Data between two groups (control andlow iron diet) were compared by Student's t-test. The statistical significance of correlation was deter-mined by the use of a simple regression analysis. A P value of 0.05 was considered to be significant.FundingThis work was supported by grants from MIUR Ministero dell’Università e Ricerca Scientifica (FIRB2012, codice unico progetto B81J12003390001: RBFR12NSCF-001, RBFR12NSCF-002, RBFR12NSCF-003).AcknowledgmentsWe are deeply grateful to Marco Tripodi, Riccardo Alessandro and Nicola Perrotti for their scientificsupport.Transparency document. Supporting informationTransparency data associated with this article can be found in the online version at http://dx.doi.org/10.1016/j.dib.2017.03.020.References[1] T. Furutani, K. Hino, M. Okuda, T. Gondo, S. Nishina, A. Kitase, M. Korenaga, S.Y. Xiao, S.A. Weinman, S.M. Lemon, I. Sakaida,K. Okita, Hepatic iron overload induces hepatocellular carcinoma in transgenic mice expressing the hepatitis C viruspolyprotein, Gastroenterology 130 (2006) 2087–2098.[2] S. Nishina, M. Korenaga, I. Hidaka, A. Shinozaki, A. Sakai, T. Gondo, M. Tabuchi, F. Kishi, K. Hino, Hepatitis C virus protein andiron overload induce hepatic steatosis through the unfolded protein response in mice, Liver Int. 30 (2010) 683–692.[3] L. Serfaty, T. Andreani, P. Giral, N. Carbonell, O. Chazouillères, R. Poupon, Hepatitis C virus induced hypobetalipoprotei-nemia: a possible mechanism for steatosis in chronic hepatitis C, J. Hepatol. 34 (2001) 428–434.[4] C. Mancone, C. Montaldo, L. Santangelo, C. Di Giacomo, V. Costa, L. Amicone, G. Ippolito, L.P. Pucillo, T. Alonzi, M. Tripodi,Ferritin heavy chain is the host factor responsible for HCV-induced inhibition of apoB-100 production and is required forefficient viral infection, J. Proteome Res. 11 (2012) 2786–2797.[5] A. Blindenbacher, F.H. Duong, L. Hunziker, S.T. Stutvoet, X. Wang, L. Terracciano, D. Moradpour, H.E. Blum, T. Alonzi,M. Tripodi, N. La Monica, M.H. Heim, Expression of hepatitis c virus proteins inhibits interferon alpha signaling in the liverof transgenic mice, Gastroenterology 124 (2003) 1465–1475.

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Data on the effects of low iron diet on serum lipid profile in HCV transgenic mouse model

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