The effect of nerolidol and farnesol on the selected hepatic biotransformation enzymes

Abstract

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Diana Dimunová Supervisor: Prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The effect of nerolidol and farnesol on the selected hepatic biotransformation enzymes Sesquiterpenes represent a class of terpenoids that are mostly produced as secondary metabolites in higher plants. These compounds play an important role as components of many folk medicines and dietary supplements. In recent years, they have been studied in view of their antioxidant, anti-parasitic and anti-carcinogenic activities. On the other hand, sesquiterpenes could change the activity of biotransformation enzymes and by this way affect the metabolism of drugs, the safety and efficiency of pharmacotherapy. The aim of diploma thesis was to find out the influence of three acyclic sesquiterpenes farnesol (FAR), cis- nerolidol (CNER) and trans-nerolidol (TNER) on activity and expresion of reducing enzymes (aldehyd reductase AKR1A1, carbonyl reductase CBR1, NADH-quinone oxidoreductase1 NQO1) and conjugating enzymes (sulfotransferase SULT, glutathione-S-transferase GST, UDP-glucuronosyltransferase UGT). The potential inhibitory effects of sesquiterpenes were tested in hepatic subcellular fractions, while primary..