Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Lenka Rejšková Supervisor: doc. Ing. Barbora Szotáková, Ph.D. Consultant: Mgr. Lukáš Prchal Title of rigorous thesis: Biotransformation enzymes and metabolism of drugs in Fascioloides magna Biotransformation of xenobiotics including drugs is detoxifying ability of all organisms and it leads to positive and/or negative effects of pharmacotherapy. Each animal species is distinguished by quantitative and qualitative differences in biotransformation enzymes. Knowledge of these differences is crucial for effective and safe pharmacotherapy. It has become vital particularly when drug resistance is becoming a worldwide problem. This study was focused on parasite species Fascioloides magna which is responsible for extensive deaths of ruminants. In spite of this fact, there is lack of detailed information on its metabolism of anthelmintics or metabolism in general. The aim of this study was to find out biotransformation of anthelmintics in F. magna and activity of biotransformation enzymes in subcellular fractions from F. magna in the presence of model substrates. On the basis of fact that there were obtained activities of oxidative enzymes (FMO, GPx, CAT, Px, TrxR and NQO-1) and conjugation..
