T- and B- lymphocytes play a key role in immunity, because they provide specific immunity in organism. Receptor S1P1 regulates lymphocyte egress from lymphoid organs to blood, from which the lymphocytes travel to the site of infection. It was discovered that transmembrane helix 4 of S1P1 receptor interacts with transmembrane part of CD69 receptor and this interaction is responsible for retention of lymphocytes in lymphoid organs. The aim of this study is to prepare expression plasmids for production of these two receptors and their further research
